血管细胞粘附分子1(VCAM1)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Vascular Cell Adhesion Molecule 1 (VCAM1)
CD106; INCAM-100; L1CAM
- 编号USEA547Si
- 物种Rhesus monkey (Simian，恒河猴) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围3.9-250ng/mL
- 灵敏度最小可检测剂量小于等于1.5ng/mL.
- 样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 1701 ¥ 2430 ¥ 10935 ¥ 20655 ¥ 170100
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测血管细胞粘附分子1(VCAM1)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的血管细胞粘附分子1(VCAM1)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 91-98 | 95 |
| EDTA plasma(n=5) | 98-105 | 102 |
| heparin plasma(n=5) | 78-92 | 88 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的血管细胞粘附分子1(VCAM1)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中血管细胞粘附分子1(VCAM1)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 99-105% | 80-102% | 87-99% | 84-98% |
| EDTA plasma(n=5) | 84-101% | 97-105% | 89-97% | 78-97% |
| heparin plasma(n=5) | 98-105% | 80-101% | 84-95% | 97-104% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将血管细胞粘附分子1(VCAM1)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的血管细胞粘附分子1(VCAM1)与连接于固相载体上的抗体结合，然后加入生物素化的血管细胞粘附分子1(VCAM1)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的血管细胞粘附分子1(VCAM1)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Rhesus monkey (Simian，恒河猴) | 应用(仅供研究使用，不用于临床诊断！) |
| URPA547Si01 | 血管细胞粘附分子1(VCAM1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA547Si01 | 血管细胞粘附分子1(VCAM1)多克隆抗体 | WB; IHC |
| UMAA547Si21 | 血管细胞粘附分子1(VCAM1)单克隆抗体 | WB; IHC; ICC; IP. |
| USEA547Si | 血管细胞粘附分子1(VCAM1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA547Si | 血管细胞粘附分子1(VCAM1)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Amran et al. Lipids in Health and Disease | Changes in the vascular cell adhesion molecule-1, intercellular adhesion molecule-1 and c-reactive protein following administration of aqueous extract of piper sarmentosum on experimental. [BioMed: 1476511X] |
| Vascular Pharmacology | Long-term ethanol consumption initiates atherosclerosis in rat aorta through inflammatory stress and endothelial dysfunction [ScienceDirect: S1537189112000705] |
| Canadian Journal of Physiology and Pharmacology | Resveratrol abrogates adhesion molecules and protects against TNBS-induced ulcerative colitis in rats. [PubMed: 22029500] |
| British Journal of Medical and Health Sciences | EFFECTS OF ADRIAMYCIN, CISPLATIN, AND 5-FLUOROURACIL ON THE TESTES OF ALBINO RATS [Researchgate: Source] |
| Endocrine Metabolic & Immune Disordors Drug Targets. | Active immunization against tumor necrosis factor-alpha decreases proinflammatory cytokines, oxidative stress mediators and adhesion molecules risk factors in streptozotocin-induced diabetic rats. [Pubmed: 23859636] |
| Atherosclerosis | Increased monocyte adhesion by endothelial expression of VCAM-1 missense variation in vitro [Pubmed: 24075742] |
| Atherosclerosis | Activation of receptor for advanced glycation end products contributes to aortic remodeling and endothelial dysfunction in sinoaortic denervated rats. [Pubmed: 23880178] |
| BioMed research international | Endothelial gene expression and molecular changes in response to radiosurgery in in vitro and in vivo models of cerebral arteriovenous malformations [Pubmed: 24199192] |
| Arch Dis Child | PO-0699 Estimation Of Adhesion Molecules Intrauterine Growth Retardation (iugr) Newborns [Bmj:Source] |
| Arch Dis Child? | PO-0701 Causes Of Fetal Growth Restriction And Early Neonatal Outcome [Bmj:Source] |
| Arch Dis Child | PO-0698 Neonatal Resuscitation Practices In The Delivery Room In India: An Audit Using Videography [Bmj:Source] |
| Naunyn-Schmiedeberg's Archives of Pharmacology | Insights into the antiatherogenic molecular mechanisms of andrographolide against?Porphyromonas gingivalis-induced atherosclerosis in rabbits [Pubmed:25172523] |
| Nutr Res. | Curcumin attenuates adhesion molecules and matrix metalloproteinase expression in hypercholesterolemic rabbits [Pubmed:25282128] |
| Alexandria Journal of Medicine | Effect of consumption of fresh and heated virgin coconut oil on the blood pressure and inflammatory biomarkers: An experimental study in Sprague Dawley rats [Sciencedirect:S2090506814000268] |
| Canadian Journal of Physiology and Pharmacology | Effect of magnesium sulfate and thyroxine on inflammatory markers in a rat model of hypothyroidism [Doi: Abs] |
| Hum Exp Toxicol | Effect of agmatine on experimental vascular endothelial dysfunction [PubMed: 26424770] |
| International Journal of Food Sciences and Nutrition | Serum lipid profile and inflammatory markers in the aorta of cholesterol-fed rats supplemented with extra virgin olive oil, sunflower oils and oil-products [PubMed: 26401576] |
| J Trace Elem Med Biol | Comparative angioprotective effects of magnesium compounds [PubMed: 25127069] |
| J Mol Cell Cardiol | Diverse contribution of bone marrow-derived late-outgrowth endothelial progenitor cells to vascular repair under pulmonary arterial hypertension and arterial neointimal formation [PubMed: 26231083] |
| Endocrine | Effects of glucagon-like peptide-1 on advanced glycation endproduct-induced aortic endothelial dysfunction in streptozotocin-induced diabetic rats: possible roles of Rho kinase- and AMP kinase-mediated nuclear factor κB signaling pathways [Pubmed:26758998] |
| Mol Neurobiol | A Therapeutic Insight of Niacin and Coenzyme Q10 Against Diabetic Encephalopathy in Rats [Pubmed:26867655] |
| Pakistan journal of pharmaceutical sciences | Changes in blood pressure, vascular reactivity and inflammatory biomarkers following consumption of heated corn oil. [pubmed:29084680] |
| Acta Biochim Biophys Sin | Therapeutic potential of mesenchymal stem cells in acute kidney injury is affected by administration timing [pubmed:28338909] |
| Experimental and Therapeutic Medicine | Epigallocatechin‑3‑gallate attenuates neointimal hyperplasia in a rat model of carotid artery injury by inhibition of high mobility group box 1 expression [pubmed:28962112] |
| Experimental Parasitology | Adhesion molecules, chemokines and matrix metallo-proteinases response after albendazole and albendazole plus steroid therapy in swine neurocysticercosis [pubmed:28821422] |
| Evidence-Based Complementary and Alternative Medicine | BuShenKangShuai tablet reduces atherosclerotic lesion by improving blood lipids metabolism and inhibiting inflammatory response via TLR4 and NF-κB signaling pathway [journals:1758383] |
| Journal of Chromatography A | Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis [S0021967317311858] |
| International Journal of Clinical and Experimental Pathology | Protective effect of high mobility group box-1 silence on diabetic retinopathy: an in vivo study [ISSN:1936-2625/IJCEP0055881] |
| ResearchGate | Significance of Soluble Endoglin as a Possible Marker of Cardiovascular Disease in Type 2 Diabetic Patients [315113405] |
| Regenerative Biomaterials | The directional migration and differentiation of mesenchymal stem cells toward vascular endothelial cells stimulated by biphasic calcium phosphate ceramic [10.1093/rb/rbx028] |
| Anesthesiology | Vasculotide, an angiopoietin-1 mimetic, restores microcirculatory perfusion and microvascular leakage and decreases fluid resuscitation requirements in … [Pubmed:28968277] |
| PLoS Neglected Tropical Diseases | Characterization of the rhesus macaque (Macaca mulatta) scrub typhus model: Susceptibility to intradermal challenge with the human pathogen Orientia … [Pubmed:29522521] |
| Life Sciences | Nephroprotective effect of saxagliptin against gentamicin-induced nephrotoxicity, emphasis on anti-oxidant, anti-inflammatory and anti-apoptic effects [Pubmed:30012474] |
| Evidence-based Complementary and Alternative Medicine | Bushenkangshuai Tablet Reduces Atherosclerotic Lesion by Improving Blood Lipids Metabolism and Inhibiting Inflammatory Response via TLR4 and NF-κB Signaling … [Pubmed:29619063] |
| Nutrition & Metabolism | L-Carnitine Supplementation Increases Trimethylamine-N-Oxide but not Markers of Atherosclerosis in Healthy Aged Women [Pubmed: 30485835] |
| Cytokine | Intercellular adhesion molecule-1 (ICAM-1) associates with 24-hour ambulatory blood pressure variability in type 2 diabetes and controls [Pubmed: 30716657] |
| Anticancer Research | Effect of Radiation on the Expression of CVD-related miRNAs, Inflammation and Endothelial Dysfunction of HUVECs [Pubmed: 30711956] |
| European Journal of Pharmacology | The cordycepin derivative IMM-H007 improves endothelial dysfunction by suppressing vascular inflammation and promoting AMPK-dependent eNOS activation in … [Pubmed: 30826323] |
| international journal of molecular sciences | Anti-Inflammatory and Anti-Apoptotic Effects of Stybenpropol A on Human Umbilical Vein Endothelial Cells [Pubmed: 31671764] |
| Archives of Insect Biochemistry and Physiology | Vanadyl sulphate ameliorates biomarkers of endothelial injury and coagulation and thrombosis in a rat model of hyperglycaemia [Pubmed: 31774317] |
| BMC Endocrine Disorders | The role of the adipocytokines vaspin and visfatin in vascular endothelial function and insulin resistance in obese children [Pubmed: 31771561] |
| Scientific Reports | Association between Upper-airway Surgery and Ameliorative Risk Markers of endothelial function in obstructive Sleep Apnea [Pubmed: 31882827] |
| BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE | Endothelial epithelial sodium channel involves in high-fat diet-induced atherosclerosis in low-density lipoprotein receptor-deficient mice [Pubmed: 33065235] |
| Antioxidants (Basel) | Anti-Atherogenic Effects of Orlistat on Obesity-Induced Vascular Oxidative Stress Rat Model [33562069] |
| Sci. Pharm | Dehydroepiandrosterone (DHEA) Improves the Metabolic and Haemostatic Disturbances in Rats with Male Hypogonadism [] |
| Journal of the South African Veterinary Association | Circulating markers of endothelial activation in canine parvoviral enteritis [] |
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