促甲状腺素(TSH)等多因子检测试剂盒(流式荧光发光法)

Multiplex Assay Kit for Thyroid Stimulating Hormone (TSH) ,etc. by FLIA (Flow Luminescence Immunoassay)

Thyrotropin

（注：单次混测多因子不超过8个指标 ）

  • 促甲状腺素(TSH)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • 促甲状腺素(TSH)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测促甲状腺素(TSH)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的促甲状腺素(TSH)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 80-90 85
EDTA plasma(n=5) 87-94 91
heparin plasma(n=5) 86-101 92

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

线性

在定值血清及血浆样本内加入适量的促甲状腺素(TSH)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中促甲状腺素(TSH)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 99-105% 86-104% 93-101% 81-89%
EDTA plasma(n=5) 87-96% 94-103% 97-104% 88-104%
heparin plasma(n=5) 80-101% 93-101% 92-101% 85-92%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠、检测溶液A）标准品或样本50μL及磁珠10μL，加检测溶液A50μL，
    37°C酶标板振荡器孵育60分钟；
3. 磁吸洗板3次；
4. 加检测溶液B100μL，37°C振动孵育30分钟；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋涡震荡2分钟后读数。

实验原理

将促甲状腺素(TSH)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠、标记VEGFA，标记的促甲状腺素(TSH)等多因子检测试剂盒(流式荧光发光法)和未标记的促甲状腺素(TSH)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体竞争结合，然后将未结合物洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的促甲状腺素(TSH)等多因子检测试剂盒(流式荧光发光法)呈负相关。

赠品

相关产品

编号 适用物种：Bos taurus; Bovine (Cattle，牛) 应用(仅供研究使用，不用于临床诊断！)
CEA463Bo 促甲状腺素(TSH)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA463Bo 促甲状腺素(TSH)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Blood Alterations in the adenosine metabolism and CD39/CD73 adenosinergic machinery cause loss of Treg cell function and autoimmunity in ADA-deficient SCID [Pubmed: 22184407]
Life Science Journal Can Nigella Sativa oil (NSO) reverse hypothyroid status induced by PTU in rat? biochemical and histological studies [Lifesciencesite: Source]
Thyroid Impact of Metformin and Compound C on NIS expression and iodine uptake in vitro and in vivo: a role for CRE in AMPK modulation of thyroid function [Pubmed: 23819433]
Journal of Functional Foods Effect of lettuce biofortified with iodine by soil fertilization on iodine concentration in various tissues and selected biochemical parameters in serum of Wistar rats [Sciencedirect:S1756464615000894]
J Endocrinol. Thyroid status modulates T lymphoma growth via cell cycle regulatory proteins and angiogenesis [Pubmed:24928937]
journal of lipid research Thyroid-stimulating hormone decreases HMG-CoA reductase phosphorylation via AMP-activated protein kinase in the liver [PubMed: 25713102]
Int J Clin Exp Med Lead exposure causes thyroid abnormalities in diabetic rats [PubMed: 26221254]
Jubilant Drax Image Perinatal Iron Deficiency-Induced Hypothyroxinemia Impairs Early Brain Development Regardless of Normal Iron Levels in the Neonatal Brain [Pubmed:27231981]
PLoS One. The Impact of Carrot Enriched in Iodine through Soil Fertilization on Iodine Concentration and Selected Biochemical Parameters in Wistar Rats [Pubmed:27043135]
journal of bone and mineral research Knock-In of the Recurrent R368X Mutation of PRKAR1A that Represses cAMP-Dependent Protein Kinase A Activation: A Model of Type 1 Acrodysostosis. [pubmed:27589370]
Toxicological Sciences Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspring [pubmed:27803384]
Sci Rep Exposure of pregnant mice to triclosan impairs placental development and nutrient transport. [pubmed:28322267]
Bulletin of the World Health Organization Endemic goitre in the Sudan despite longstanding programmes for the control of iodine deficiency disorders [handle:123456789]
International Journal of Peptide Research and Therapeutics Effect of Intracerebroventricular Administration of Apelin-13 on the Hypothalamus–Pituitary–Thyroid Axis and Peripheral Uncoupling Proteins [article:10.1007]
Tropical Journal of Pharmaceutical Research Neuroprotective effects of thymoquinone against cerebellar histopathological changes in propylthiouracil-induced hypothyroidism in adult rats [10.4314/tjpr.v16i5.9]
30 The NANCI-Nkx2.1 gene duplex buffers Nkx2.1 expression to maintain lung development and homeostasis. [pubmed:28546511]
NITRIC OXIDE-BIOLOGY AND CHEMISTRY Voluntary exercise training restores anticontractile effect of NO in coronary arteries of adult rats with antenatal/early postnatal hypothyroidism [Pubmed:29307633]
Environmental Pollution Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population [Pubmed:29525625]
Frontiers in Molecular Neuroscience Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice [Pubmed:29403355]
Neuroscience Letter Central irisin administration suppresses thyroid hormone production but increases energy consumption in rats [Pubmed:29574218]
Journal of Neuroinflammation Hashimoto's thyroiditis induces neuroinflammation and emotional alterations in euthyroid mice [Pubmed: 30373627]
Cell Reports Zika Virus Infection in Hypothalamus Causes Hormone Deficiencies and Leads to Irreversible Growth Delay and Memory Impairment in Mice [Pubmed: 30404008]
Journal of Diabetes Research The Adverse Effects of Thyrotropin Absence on Pancreatic β Cell Function in Mice []
Endocrine Hypothyroidism-related zinc deficiency leads to suppression of T lymphocyte activity [Pubmed: 31056723]
Oxidative Medicine and Cellular Longevity Oxidative Stress Produced by Hyperthyroidism Status Induces the Antioxidant Enzyme Transcription through the Activation of the Nrf-2 Factor in Lymphoid Tissues of … []
Reproductive Biology and Endocrinology Hashimoto's thyroiditis impairs embryo implantation by compromising endometrial morphology and receptivity markers in euthyroid mice [Pubmed: 31729993]
Gli Tanycyte ablation in the arcuate nucleus and median eminence increases obesity susceptibility by increasing body fat content in male mice [Pubmed: 32173924]
Clinical Immunology The role of protein disulphide-isomerase A3 as autoantigen in the pathogenesis of autoimmune thyroiditis and related brain damage in adult mice [Pubmed: 31982645]
Nature Feeding induces cholesterol biosynthesis via the mTORC1–USP20–HMGCR axis [Pubmed: 33177714]
Thyroid Generation and Characterization of a New Resistance to Thyroid Hormone Mouse Model with Thyroid Hormone Receptor Alpha Gene Mutation [Pubmed: 32924834]
Thyroid Hashimoto's thyroiditis induces hippocampus-dependent cognitive alterations by impairing astrocytes in euthyroid mice [Pubmed: 32907517]
Int Immunopharmacol Experimental evidence for alpha enolase as one potential autoantigen in the pathogenesis of both autoimmune thyroiditis and its related encephalopathy [Pubmed: 32442899]
Brazilian Journal of Development Maternal exposure of triclosan causes fetal development restriction and female reproductive alterations in rat offspring/A exposição materna ao triclosan causa … []
Comparaison des marqueurs hormonaux et histomorphométriques chez la souris un modèle d'hypothyroïdie après 28 jours de traitement au PTU par voie alimentaire. []
Andrologia Thyroid hormones, Sertoli cell proliferation and differentiation in progenies from carbamazepine©\treated rat dams during pregnancy and lactation [33433934]
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Interplay between Thyroid Hormones and Stearoyl-CoA Desaturase 1 in the Regulation of Lipid Metabolism in the Heart [33375430]
Biomedicines Role of Kallikrein 7 in Body Weight and Fat Mass Regulation. Biomedicines 2021, 9, 131 [33572949]
Mol Neurobiol Maternal Subclinical Hypothyroidism in Rats Impairs Spatial Learning and Memory in Offspring by Disrupting Balance of the TrkA/p75 NTR Signal Pathway [33966253]
Clin Epigenetics Genome?wide profiling of DNA methylation and gene expression unravel the epigenetic landscape in diabetes-related hypothyroidism [34092241]
Pharmaceutics Improved Therapeutic Efficiency against Obesity through Transdermal Drug Delivery Using Microneedle Arrays []
BRITISH JOURNAL OF NUTRITION Effects of iodine excess on serum thyrotropin-releasing hormone levels and type 2 deiodinase in the hypothalamus of Wistar rats [34250878]
Gene Therapy Correction of a knock-in mouse model of acrodysostosis with gene therapy using a rAAV9-CAG-human PRKAR2A vector [34599290]
NatCommun Plasmin activity promotes amyloid deposition in a transgenic model of human transthyretin amyloidosis [34876572]
Int J Mol Sci The Deubiquitinase OTUB1 Is a Key Regulator of Energy Metabolism [Pubmed:35163456]
Molecular Biology Reports Effects of meteorin-like hormone on endocrine function of hypothalamo-hypophysial system and peripheral uncoupling proteins in rats [Pubmed:35332411]
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