三磷酸腺苷(ATP)等多因子检测试剂盒(流式荧光发光法)
Multiplex Assay Kit for Adenosine Triphosphate (ATP) ,etc. by FLIA (Flow Luminescence Immunoassay)
Adenosine-5'-Triphosphate
(注:单次混测多因子不超过8个指标 )
- 编号LMA349Ge
- 物种Pan-species (General,通用) 相同的名称,不同的物种。
- 实验方法竞争抑制
- 反应时长1.5h
- 检测范围0.98-1000ng/mL
- 灵敏度最小可检测剂量小于等于0.327 ng/mL.
- 样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 8指标数 7指标数 6指标数 5指标数 4指标数 3指标数 2指标数1指标数
- 价格 ¥ 2980 ¥ 3094 ¥ 3266 ¥ 3495 ¥ 3725 ¥ 4068 ¥ 4584 计算器 ¥ 5730 添加到价格计算器
- 欲了解实际交易价格和更多情况,请与当地经销商联系!
特异性
本试剂盒用于检测三磷酸腺苷(ATP)等多因子检测试剂盒(流式荧光发光法),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的三磷酸腺苷(ATP)等多因子检测试剂盒(流式荧光发光法)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。
样本 | 回收率范围(%) | 平均回收率(%) |
serum(n=5) | 81-104 | 95 |
EDTA plasma(n=5) | 85-98 | 88 |
heparin plasma(n=5) | 95-103 | 98 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%
线性
在定值血清及血浆样本内加入适量的三磷酸腺苷(ATP)等多因子检测试剂盒(流式荧光发光法),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中三磷酸腺苷(ATP)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。
样本 | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 83-101% | 83-93% | 86-96% | 80-101% |
EDTA plasma(n=5) | 88-97% | 89-97% | 79-101% | 94-102% |
heparin plasma(n=5) | 78-98% | 86-101% | 78-90% | 98-105% |
稳定性
经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本准备;
2. 加样(标准品、样本、磁珠、检测溶液A)标准品或样本50μL及磁珠10μL,加检测溶液A50μL,
37°C酶标板振荡器孵育60分钟;
3. 磁吸洗板3次;
4. 加检测溶液B100μL,37°C振动孵育30分钟;
5. 磁吸洗板3次;
6. 加鞘液100μL,旋涡震荡2分钟后读数。
实验原理
将三磷酸腺苷(ATP)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠,制成固相载体,向微孔中分别加入标准品或标本以及磁珠、标记VEGFA,标记的三磷酸腺苷(ATP)等多因子检测试剂盒(流式荧光发光法)和未标记的三磷酸腺苷(ATP)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体竞争结合,然后将未结合物洗净后,加入PE标记的亲和素,再次彻底洗涤后即可上机读数。MFI值和样品中的三磷酸腺苷(ATP)等多因子检测试剂盒(流式荧光发光法)呈负相关。
赠品
增值服务
相关产品
编号 | 适用物种:Pan-species (General,通用) | 应用(仅供研究使用,不用于临床诊断!) |
CPA349Ge11 | 三磷酸腺苷(ATP)牛血清白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
CPA349Ge21 | 三磷酸腺苷(ATP)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
PAA349Ge01 | 三磷酸腺苷(ATP)多克隆抗体 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
LAA349Ge81 | 三磷酸腺苷(ATP)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
LAA349Ge71 | 三磷酸腺苷(ATP)多克隆抗体(生物素标记) | WB; IHC; ICC. |
CEA349Ge | 三磷酸腺苷(ATP)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
CCA349Ge | 三磷酸腺苷(ATP)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMA349Ge | 三磷酸腺苷(ATP)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
KSA349Ge11 | 三磷酸腺苷(ATP)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
杂志 | 参考文献 |
Iranian Journal of Health and Physical activity | Effects of Aerobic Training, with or without Zizyphus Jujuba Water Extraction, on Fundus Nesfatin-1, ATP, HDL-C, and LDL-C Concentrations in Female Rats [Ijvst: Source] |
Journal of Neurochemistry | Neuroprotective effects of vildagliptin in rat rotenone Parkinson's disease model: role of RAGE‐NFκB and Nrf2‐antioxidant signaling pathways [PubMed: 25752913] |
American Journal of Animal and Veterinary Sciences | Metabolic Features of Heart Failure with Different Etiology [ofsp11075] |
toxicology in vitro | Rifampicin-induced injury in L02 cells is alleviated by 4-PBA via inhibition of the PERK-ATF4-CHOP pathway. [pubmed:27470132] |
Free radical biology and medicine | Rifampicin-induced injury in HepG2 cells is alleviated by TUDCA via increasing bile acid transporters expression and enhancing the Nrf2-mediated adaptive response. [pubmed:28688954] |
Dipòsit Digital de la Universitat de Barcelona | Chemiluminescent bioanalytical assays for clinical biomarkers [2445/116053] |
Evidence-Based Complementary and Alternative Medicine | The Antioxidative Action of ZTP by Increasing Nrf2/ARE Signal Pathway [] |
Antihypoxic and anti-ischemic properties of the North Caucasus flora plant extracts [] | |
Respiratory Physiology & Neurobiology | Effects of nanoparticles on Neuroinflammation in a Mouse Model of Asthma [Pubmed: 31542455] |
Journal of Cosmetic Dermatology | Novel complex of cosmetic ingredients with promising action in preventing hair loss and follicular aging through mechanism involving enrichment of WNT/signaling … [Pubmed: 33179848] |
Bangladesh Journal of Pharmacology | Ethylmethylhydroxypyridine succinate, acetylcysteine and choline alphoscerate improve mitochondrial function under condition of cerebral ischemia in rat [] |
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | Prostaglandin E receptor subtype 4 protects against diabetic cardiomyopathy by modulating cardiac fatty acid metabolism via FOXO1/CD36 signalling [33647796] |
Serum Metabolomic Analysis of Coronary Heart Disease Patients with Stable Angina Pectoris Subtyped by Traditional Chinese Medicine Diagnostics Reveals ¡ [] | |
Neurourol Urodyn | Estrogen inhibits bladder overactivity in rats with cyclophosphamide‐induced cystitis via downregulating the expression of P2X3 receptors in bladder epithelium cells [34622458] |
Biomed Res Int | A Famous Chinese Medicine Formula: Yinhuo Decoction Antagonizes the Damage of Corticosterone to PC12 Cells and Improves Depression by Regulating … [Pubmed:35281603] |