癌胚抗原(CEA)等多因子检测试剂盒(流式荧光发光法)

Multiplex Assay Kit for Carcinoembryonic Antigen (CEA) ,etc. by FLIA (Flow Luminescence Immunoassay)

CD66E; CD66; CEACAM5; Carcinoembryonic Antigen-related Cell Adhesion Molecule 5; Carcinoembryonic Antigen

（注：单次混测多因子不超过8个指标 ）

  • 癌胚抗原(CEA)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • 癌胚抗原(CEA)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测癌胚抗原(CEA)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的癌胚抗原(CEA)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 92-99 96
EDTA plasma(n=5) 80-104 92
heparin plasma(n=5) 92-105 97

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

线性

在定值血清及血浆样本内加入适量的癌胚抗原(CEA)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中癌胚抗原(CEA)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 88-97% 81-99% 83-99% 96-104%
EDTA plasma(n=5) 95-105% 80-89% 80-99% 96-103%
heparin plasma(n=5) 91-105% 96-104% 98-105% 87-96%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠）标准品或样本100μL及磁珠10μL，
    37°C酶标板振荡器孵育90分钟；
3. 磁吸甩干，加检测溶液A100μL，37°C酶标板振荡器孵育60分钟；
4. 磁吸洗板3次；
5. 加检测溶液B100μL，37°C振动孵育30分钟；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋涡震荡2分钟后读数。

实验原理

将癌胚抗原(CEA)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠，其中的癌胚抗原(CEA)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体结合，然后加入生物素化的癌胚抗原(CEA)等多因子检测试剂盒(流式荧光发光法)抗体，将未结合的生物素化抗体洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的癌胚抗原(CEA)等多因子检测试剂盒(流式荧光发光法)呈正相关。

赠品

相关产品

编号 适用物种：Rattus norvegicus (Rat，大鼠) 应用(仅供研究使用，不用于临床诊断！)
URPA150Ra01 癌胚抗原(CEA)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
UPAA150Ra01 癌胚抗原(CEA)多克隆抗体 WB
UMAA150Ra21 癌胚抗原(CEA)单克隆抗体 WB; IHC; ICC; IP.
ULMA150Ra 癌胚抗原(CEA)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
BioFactors Ginger ingredients inhibit the development of diethylnitrosoamine induced premalignant phenotype in rat chemical hepatocarcinogenesis model [PubMed: 20872761]
Life Sciences Comparison of angiotensin converting enzyme inhibitors and angiotensin II type 1 receptor blockade for the prevention of premalignant changes in the liver [ScienceDirect: S0024320511002700]
Iranian journal of basic medical sciences Saffron Aqueous Extract Inhibits the Chemically-induced Gastric Cancer Progression in the Wistar Albino Rat [PubMed: PMC3637902]
Biosensors and Bioelectronics Handy, rapid and multiplex detection of tumor markers based on encoded silica-hydrogel hybrid beads array chip [Pubmed: 23672876]
Asian Pac J Cancer Prev. Association of Paraoxonase-1 (Q192R and L55M) Gene Polymorphisms and Activity with Colorectal Cancer and Effect of Surgical Intervention. [Pubmed:25684529]
Journal of Analytical Atomic Spectrometry Detection of HIV-1 p24 antigen using streptavidin–biotin and gold nanoparticles based immunoassay by inductively coupled plasma mass spectrometry [Rsc:Source]
Breast Activated Leukocyte Cell Adhesion Molecule (ALCAM) in Saudi Breast Cancer Patients as Prognostic and Predictive Indicator [PubMed: 26446295]
The Journal of Toxicological Sciences Gene expression analysis in the lung of the rasH2 transgenic mouse at week 4 prior to induction of malignant tumor formation by urethane and N-methylolacrylamide [PubMed: 26558449]
PLoS One Collecting Protein Biomarkers in Breath Using Electret Filters: A Preliminary Method on New Technical Model and Human Study [Pubmed:26934615]
Journal of Applied Pharmaceutical Science Chemopreventive effect of Indigofera linnaei extract against diethylnitrosamine induced hepatocarcinogenesis in rats [2071_pdf.pdf]
Analytical chemistry Temporal Sensing Platform Based on Bipolar Electrode for the Ultrasensitive Detection of Cancer Cells [pubmed:27506255]
Frontiers in Physiology  Anticarcinogenic potential of ethanol extract of Indigofera cordifolia Roth.(Fabales: Fabaceae) on diethylnitrosamine induced hepatocarcinogenesis in rats [v04n07a07a]
Analytica Chimica Acta Ultrasensitive cathode photoelectrochemical immunoassay based on TiO2 photoanode-enhanced 3D Cu2O nanowire array photocathode and signal amplification by … [Pubmed:29866267]
Journal of Cancer Evaluation of Detection Methods and Values of Circulating Vascular Endothelial Growth Factor in Lung Cancer [Pubmed:29675110]
Clinical Breast Cancer 8-Hydroxy-2'-deoxyguanosine as a Discriminatory Biomarker For Early Detection of Breast Cancer []
Journal of Ethnopharmacology Preventive potential of dietary inclusion of Brachystegia eurycoma (Harms) seeds on N-methyl-N-nitrosourea-induced colon carcinogenesis in Wistar rats [Pubmed: 30953819]
Asian Journal of Pharmacy and Pharmacology Chemopreventive effects of on diethylnitrosamine induced and Indigofera cassioides phenobarbital promoted rat liver carcinoma []
Biomaterials DNA-templated porous nanoplatform towards programmed “double-hit” cancer therapy via hyperthermia and immunogenicity activation [Pubmed: 31374481]
Biology Piperine Regulates Nrf-2/Keap-1 Signalling and Exhibits Anticancer Effect in Experimental Colon Carcinogenesis in Wistar Rats [Pubmed: 32967203]
Oncology Research and Treatment Evaluation of the Diagnostic and Predicative Values of 8-Iso-Prostaglandin F2α as a Biomarker of Breast Cancer [Pubmed: 32721979]
Journal of Functional Foods Chemopreventive effect of dietary inclusion with Crassocephalum rubens (Juss ex Jacq) leaf on N-methyl-N-nitrosourea (MNU)-induced colorectal carcinogenesis in … []
Lateral Flow Immunosensor for Ferritin Based on Dual Signal-Amplified Strategy by Rhodium Nanoparticles []
Anti-Cancer Activity of Piperine Against Colon Carcinogenesis Via Modulation of NF-κB/Nrf-2/Keap1/HO-1 Signalling Pathways []
Microchemical Journal Magnetic immunoassay for tumor clinical diagnosis based on rolling circular amplification (RCA) coupled with ICP-MS []
Anticancer efficacy of β-Sitosterol Loaded Hydroxyapatite-Alginate on Colon Cancer Cell in Vivo []
sensors and actuators b-chemical A light-induced self-powered competitive immunosensor for the detection of platelet derived growth factor-BB via an elaborately assembled bioconjugate []
留言咨询