基质Gla蛋白(MGP)等多因子检测试剂盒(流式荧光发光法)

Multiplex Assay Kit for Matrix Gla Protein (MGP) ,etc. by FLIA (Flow Luminescence Immunoassay)

MGLAP; GIG36; Cell growth-inhibiting gene 36 protein

（注：单次混测多因子不超过8个指标 ）

  • 基质Gla蛋白(MGP)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • 基质Gla蛋白(MGP)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测基质Gla蛋白(MGP)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的基质Gla蛋白(MGP)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 78-104 89
EDTA plasma(n=5) 86-93 90
heparin plasma(n=5) 79-96 89

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

线性

在定值血清及血浆样本内加入适量的基质Gla蛋白(MGP)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中基质Gla蛋白(MGP)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 91-98% 81-91% 80-97% 90-104%
EDTA plasma(n=5) 94-101% 95-104% 94-101% 80-96%
heparin plasma(n=5) 78-97% 78-97% 91-105% 93-101%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠）标准品或样本100μL及磁珠10μL，
    37°C酶标板振荡器孵育90分钟；
3. 磁吸甩干，加检测溶液A100μL，37°C酶标板振荡器孵育60分钟；
4. 磁吸洗板3次；
5. 加检测溶液B100μL，37°C振动孵育30分钟；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋涡震荡2分钟后读数。

实验原理

将基质Gla蛋白(MGP)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠，其中的基质Gla蛋白(MGP)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体结合，然后加入生物素化的基质Gla蛋白(MGP)等多因子检测试剂盒(流式荧光发光法)抗体，将未结合的生物素化抗体洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的基质Gla蛋白(MGP)等多因子检测试剂盒(流式荧光发光法)呈正相关。

赠品

相关产品

编号 适用物种：Rattus norvegicus (Rat，大鼠) 应用(仅供研究使用，不用于临床诊断！)
RPB477Ra02 基质Gla蛋白(MGP)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPB477Ra01 基质Gla蛋白(MGP)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB477Ra02 基质Gla蛋白(MGP)多克隆抗体 WB; IHC; ICC; IP.
PAB477Ra01 基质Gla蛋白(MGP)多克隆抗体 WB; IHC; ICC; IP.
LAB477Ra71 基质Gla蛋白(MGP)多克隆抗体(生物素标记) WB; IHC; ICC.
LAB477Ra81 基质Gla蛋白(MGP)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
SEB477Ra 基质Gla蛋白(MGP)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB477Ra 基质Gla蛋白(MGP)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSB477Ra01 基质Gla蛋白(MGP)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Nephrology Dialysis Transplantation Magnesium prevents phosphate-induced calcification in human aortic vascular smooth muscle cells [PubMed: PMC3611891]
Maturitas. Bone metabolism regulators and arterial stiffness in postmenopausal women [Pubmed: 23916080]
The Egyptian Rheumatologist Osteoprotegerin (OPG) and Matrix Gla protein (MGP) in rheumatoid arthritis patients: Relation to disease activity [ScienceDirect: S1110116414000076]
Toxicology Reports Canola and hydrogenated soybean oils accelerate ectopic bone formation induced by implantation of bone morphogenetic protein in mice [Researchgate:Source]
Lipids in Health and Disease Markers of increased cardiovascular risk in patients with chronic kidney disease [Biomedcentral:Source]
Human &amp; Veterinary Medicine The behavior of circulating matrix Gla protein, matrix metalloproteinase-9 and nitrotyrosine in patients with varicose veins [Hvm:Source]
Pol Arch Med Wewn Effect of Vitamin K2 on progression of atherosclerosis and vascular calcification in nondialyzed patients with chronic kidney disease stages 3-5 [PubMed: 26176325]
Lipids Health Dis. Markers of increased atherosclerotic risk in patients with chronic kidney disease: a preliminary study [pmc:PMC4739105]
Kidney Blood Press Res. Plasma Desphospho-Uncarboxylated Matrix Gla Protein as a Marker of Kidney Damage and Cardiovascular Risk in Advanced Stage of Chronic Kidney Disease [Pubmed:27100101]
Archives of Oral Biology Matrix-Gla Protein rs4236 [A/G] gene polymorphism and serum and GCF levels of MGP in patients with subgingival dental calculus [pubmed:27348051]
Cell Death & Disease Mesenchymal stromal cells-derived matrix Gla protein contribute to the alleviation of experimental colitis [Pubmed:29880866]
Urology High concentration of calcium promotes mineralization in NRK52E cells via inhibiting the expression of matrix Gla protein [Pubmed:29935264]
Clinica Chimica Acta Serum total matrix Gla protein: Reference interval in healthy adults and variations in patients with vascular and osteoarticular diseases [Pubmed: 30597137]
Biological Trace Element Research Altered Mineral Metabolism and Disequilibrium Between Calcification Promoters and Inhibitors in Chronic Hemodialysis Patients [Pubmed: 30847765]
Renal Failure Relationship of matrix Gla protein and vitamin K with vascular calcification in hemodialysis patients [Pubmed: 31538831]
Heliyon Matrix Gla protein maintains normal and malignant hematopoietic progenitor cells by interacting with bone morphogenetic protein-4 [Pubmed: 32322728]
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