摄食抑制因子1(NES1)等多因子检测试剂盒(流式荧光发光法)

Multiplex Assay Kit for Nesfatin 1 (NES1) ,etc. by FLIA (Flow Luminescence Immunoassay)

（注：单次混测多因子不超过8个指标 ）

  • 摄食抑制因子1(NES1)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • 摄食抑制因子1(NES1)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测摄食抑制因子1(NES1)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的摄食抑制因子1(NES1)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 78-104 89
EDTA plasma(n=5) 82-95 85
heparin plasma(n=5) 79-88 83

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

线性

在定值血清及血浆样本内加入适量的摄食抑制因子1(NES1)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中摄食抑制因子1(NES1)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 96-105% 83-105% 80-94% 87-97%
EDTA plasma(n=5) 82-91% 79-104% 84-101% 82-101%
heparin plasma(n=5) 97-105% 79-102% 81-101% 87-97%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠、检测溶液A）标准品或样本50μL及磁珠10μL，加检测溶液A50μL，
    37°C酶标板振荡器孵育60分钟；
3. 磁吸洗板3次；
4. 加检测溶液B100μL，37°C振动孵育30分钟；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋涡震荡2分钟后读数。

实验原理

将摄食抑制因子1(NES1)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠、标记VEGFA，标记的摄食抑制因子1(NES1)等多因子检测试剂盒(流式荧光发光法)和未标记的摄食抑制因子1(NES1)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体竞争结合，然后将未结合物洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的摄食抑制因子1(NES1)等多因子检测试剂盒(流式荧光发光法)呈负相关。

赠品

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
RPA242Hu02 摄食抑制因子1(NES1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA242Hu03 摄食抑制因子1(NES1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA242Hu01 摄食抑制因子1(NES1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
CPA242Hu21 摄食抑制因子1(NES1)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
CPA242Hu22 摄食抑制因子1(NES1)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
PAA242Hu02 摄食抑制因子1(NES1)多克隆抗体 WB; IHC; ICC; IP.
PAA242Hu08 摄食抑制因子1(NES1)多克隆抗体 WB; IHC; ICC; IP.
PAA242Hu01 摄食抑制因子1(NES1)多克隆抗体 WB; IHC; ICC; IP.
SEA242Hu 摄食抑制因子1(NES1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA242Hu 摄食抑制因子1(NES1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA242Hu 摄食抑制因子1(NES1)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA242Hu11 摄食抑制因子1(NES1)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

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Bulletin of Clinical Psychopharmacology Increased Plasma Nesfatin-1 Levels in Patients with Obsessive Compulsive Disorder [Psikofarmakoloji: 2212]
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European Journal of Obstetrics & Gynecology and Reproductive Biology Decreased serum nesfatin-1 levels in endometriosis [Pubmed: 24702902]
Bulletin of Clinical Psychopharmacology Increased Plasma Nesfatin-1 Levels in Patients with Obsessive Compulsie Disorder. [Psikofarmakoloji: Source]
Current Pharmaceutical Design Role of NUCB2/nesfatin-1 as a possible biomarker. [Pubmed: 23537082]
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Biomedicine & Pharmacotherapy Nesfatin-1 promotes VSMC migration and neointimal hyperplasia by upregulating matrix metalloproteinases and downregulating PPARγ [Pubmed:29604590]
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Probiotics and Antimicrobial Proteins Mitra Zarrati, Mahsa Raji Lahiji, Eisa Salehi, Bahareh Yazdani, Elham Razmpoosh, Raheleh Shokouhi Shoormasti & Farzad Shidfar [Pubmed: 30232744]
An elevated serum level of nesfatin-1 may predict poor outcomes in patients with aneurysmal subarachnoid hemorrhages []
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