绿色荧光蛋白(GFP)重组抗体
Recombinant Antibody to Green Fluorescent Protein (GFP)
- 编号RAD025Ge71
- 物种Pan-species (General，通用) 相同的名称，不同的物种。
- 来源重组单抗制备
- 宿主CHO
- 效价(克隆号)-
- Ig亚型 IgG
- 纯化方式蛋白A + 蛋白G亲和纯化
- 标记物生物素
- 免疫原 EPD025Ge51-绿色荧光蛋白(GFP)真核蛋白
- 缓冲液成份0.01M 磷酸盐缓冲液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性状液体
- 浓度1mg/mL
- 且适物种Pan-species (General，通用)
- 应用WB; IF; ICC; IHC; IP; FCM.
如果抗体需用于流式细胞术，请参见流式抗体。 - 下载 英文说明书 中文说明书
- 规格 20µl100µl 200µl 1ml 10ml
- 价格 ¥ 1346 ¥ 3142 ¥ 4488 ¥ 11220 ¥ 44880
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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Briefly, biotin labelled GFP Nano-antibody were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to GFP-coated microtiter wells and incubated for 1h at 37℃. Wells were washed 3 times with PBST and incubated for 0.5 h at 37℃ with SA labelled HRP. After incubation with SA labelled HRP, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of GFP and GFP Nano-antibody was shown in Figure 1, and the ED50 for this effect is 0.0187 ug/ml.
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GFP was immunoprecipitated 293F whole cell extract (Expressing GFP) with 2 µg of RAD025Ge71 and 20 µl of Nickel pillar magnetic beads ( ).
Western blot was perfomed on the immunoprecipitate using 0.05µg/ml MAD025Ge21 and 0.2µg/mL SAA544Mu19.
Lane1 (input): 293F whole cell lysate.
Lane2 ( ): RAD025Ge71 in 293F whole cell lysate.
Lane3 (-): No antibody was added to the 293F whole cell lysate.
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通过ISO 9001、ISO 13485质量体系认证
特异性
该抗体是针对GFP的小鼠单克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别GFP。
用法
Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
储存
经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
赠品
相关产品
| 编号 | 适用物种：Pan-species (General，通用) | 应用(仅供研究使用，不用于临床诊断！) |
| EPD025Ge51 | 绿色荧光蛋白(GFP)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPD025Ge01 | 绿色荧光蛋白(GFP)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPD025Ge02 | 绿色荧光蛋白(GFP)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAD025Ge07 | 绿色荧光蛋白(GFP)多克隆抗体 | WB; IHC; ICC; IP. |
| PAD025Ge02 | 绿色荧光蛋白(GFP)多克隆抗体 | WB; IHC; ICC; IP. |
| PAD025Ge51 | 绿色荧光蛋白(GFP)多克隆抗体 | WB; IHC; ICC; IP. |
| MAD025Ge21 | 绿色荧光蛋白(GFP)单克隆抗体 | WB; IHC; ICC; IP. |
| MAD025Ge22 | 绿色荧光蛋白(GFP)单克隆抗体 | WB; IHC; ICC; IP. |
| RAD025Ge71 | 绿色荧光蛋白(GFP)重组抗体 | WB; IF; ICC; IHC; IP; FCM. |
| MAD025Ge51 | 绿色荧光蛋白(GFP)单克隆抗体 | WB; IHC; ICC; IP. |
| SED025Ge | 绿色荧光蛋白(GFP)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| AED025Ge | 抗绿色荧光蛋白抗体(Anti-GFP)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
| LMD025Ge | 绿色荧光蛋白(GFP)等多因子检测试剂盒(流式荧光发光法) | Magnetic Luminex Assay for Antigen Detection. |
| KSD025Ge01 | 绿色荧光蛋白(GFP)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Explore the activation efficiency of different ligand carriers on synNotch-based contact-dependent activation system [] | |
| FASEB J | CircPrkcsh, a circular RNA, contributes to the polarization of microglia towards the M1 phenotype induced by spinal cord injury and acts via the JNK/p38 MAPK … [34751973] |
| Microbiol Spectr | Japanese Encephalitis Virus NS4A Protein Interacts with PTEN-Induced Kinase 1 (PINK1) and Promotes Mitophagy in Infected Cells [Pubmed:35604158] |
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