沉默调节蛋白1(SIRT1)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Sirtuin 1 (SIRT1)

SIR2L1; Silent Mating Type Information Regulation 2 Homolog 1; NAD-Dependent Deacetylase Sirtuin-1; SIR2-like protein 1; SirtT1 75 kDa fragment

  • 沉默调节蛋白1(SIRT1)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 沉默调节蛋白1(SIRT1)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对沉默调节蛋白1(SIRT1)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

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参考文献

杂志 参考文献
PLoS One. SIRT1 Gene Polymorphisms Affect the Protein Expression in Cardiovascular Diseases [Plos: Source]
PLOS ONE A Novel Role for SIRT-1 in L-Arginine Protection against STZ Induced Myocardial Fibrosis in Rats [Pubmed:25501750]
Journal of Alzheimer's Disease Serum miR-206 and miR-132 as Potential Circulating Biomarkers for Mild Cognitive Impairment [Pubmed:25589731]
Respirology Increases in peripheral SIRT1: A new biological characteristic of asthma [PubMed: 26040995]
PLOS ONE A Remarkable Age-Related Increase in SIRT1 Protein Expression against Oxidative Stress in Elderly: [PubMed: 25785999]
Endocrine Changes of serum Mir-217 and the correlation with the severity in type 2 diabetes patients with different stages of diabetic kidney disease. [pubmed:27522360]
Naunyn Schmiedebergs Arch Pharmacol The potential curative effect of rebamipide in hepatic ischemia/reperfusion injury [pubmed:28361247]
 International journal of cardiology A sirtuin 1/MMP2 prognostic index for myocardial infarction in patients with advanced coronary artery disease [pubmed:28043667]
International Journal of Cardiology Serum concentrations and gene expression of sirtuin 1 in healthy and slightly overweight subjects after caloric restriction or resveratrol supplementation: A randomized trial [pubmed:28029409]
Hepatology International Association of SIRT1 gene polymorphism and its expression for the risk of alcoholic fatty liver disease in the Han population [pubmed:29189974]
Toxicology and Applied Pharmacology Sildenafil protects against bile duct ligation induced hepaticfibrosis in rats: Potential role for silent information regulator 1 (SIRT1) [pubmed:28974454]
Chemico-biological Interactions Magnolol protects pancreatic β-cells against methylglyoxal-induced cellular dysfunction [pubmed:28919305]
Nutrients Gene Expression of Sirtuin-1 and Endogenous Secretory Receptor for Advanced Glycation End Products in Healthy and Slightly Overweight Subjects after … [Pubmed:30037068]
Experimental Gerontology Specific alterations in the circulating levels of the SIRT1, TLR4, and IL7 proteins in patients with dementia [Pubmed:30071285]
Kidney and Blood Pressure Research The Kidney Specific Protein myo-Inositol Oxygenase, a Potential Biomarker for Diabetic Nephropathy [Pubmed: 30504713]
Journal of International Medical Research Serum and follicular fluid levels of sirtuin 1, sirtuin 6, and resveratrol in women undergoing fertilization: an observational, clinical study [Pubmed: 30556451]
BioMed Research International Centella asiatica Prevents Increase of Hippocampal Tumor Necrosis Factor-Independently of Its Effect on Brain-Derived Neurotrophic Factor in Rat Model of Chronic … []
学位论文 Influência do tratamento da doença periodontal na sirtuína 1 e na lectina ligadora de manose em indivíduos com doença arterial coronária []
Journal of Physics D: Applied Physics Premature Myocardial Infarction: Genetic Variations in SIRT1 Affect Disease Susceptibility []
Journal of Geriatric Oncology Serum biomarkers for predicting overall survival and early mortality in older patients with metastatic solid tumors [Pubmed: 30952517]
Biomed Research International Prevents Increase of Hippocampal Tumor Necrosis Factor-α Independently of Its Effect on Brain-Derived Neurotrophic Factor in Rat Model of … [Pubmed: 30956976]
Journal of Diabetes and its Complications Vasohibin-1 in T2DM with different UACR []
Clinical Interventions in Aging The Serum Concentration of Anti-Aging Proteins, Sirtuin1 and αKlotho in Patients with End-Stage Kidney Disease on Maintenance Hemodialysis [Pubmed: 32214805]
Compr Psychiatry Crocin alleviates schizophrenia-like symptoms in rats by upregulating silent information regulator-1 and brain derived neurotrophic factor [Pubmed: 33045669]
Сибирский федеральный университет Влияние гречневой крупы на сиртуиновую активность у подопытных животных в эксперименте []
Journal of Cereal Science Dietary buckwheat enhances sirtuin1 without calorie restriction []
Clin Interv Aging Molecular Changes in Circulating microRNAs' Expression and Oxidative Stress in Adults with Mild Cognitive Impairment: A Biochemical and Molecular Study [33447019]
Antioxidants The Effect of Repeated Whole-Body Cryotherapy on Sirt1 and Sirt3 Concentrations and Oxidative Status in Older and Young Men Performing Different Levels of?¡­ [33396247]
Int Urol Nephrol Evaluation of sirtuin 1 (SIRT1) levels in autosomal dominant polycystic kidney disease [33864594]
Neurochem Int Dimethyl fumarate abridged tauo-/amyloidopathy in a D-Galactose/ovariectomy-induced Alzheimer's-like disease: Modulation of AMPK/SIRT-1, AKT/CREB/BDNF, AKT ¡­ [34052296]
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