膜联蛋白A1(ANXA1)活性蛋白

Active Annexin A1 (ANXA1)

ANX-A1; ANX1; LPC1; Lipocortin I; Chromobindin-9; Calpactin II; Phospholipase A2 inhibitory protein

  • 膜联蛋白A1(ANXA1)活性蛋白 产品包装(模拟)
  • 膜联蛋白A1(ANXA1)活性蛋白 产品包装(模拟)
  • APE787Mu01.png Figure. SDS-PAGE
  • 膜联蛋白A1(ANXA1)活性蛋白 Figure. Western Blot
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Annexin A1 (ANXA1), also known as lipocortin I, belongs to the annexin family of Ca2 -dependent phospholipid-binding proteins that have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin A1 protein has an apparent relative molecular mass of 40kDa with phospholipase A2 inhibitory activity. Besides, S100 Calcium Binding Protein A11 (S100A11) has been identified as an interactor of ANXA1, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse ANXA1 and recombinant mouse S100A11. Briefly, ANXA1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to S100A11-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-ANXA1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of ANXA1 and S100A11 was shown in Figure 1, and this effect was in a dose dependent manner.
Figure. The binding activity of ANXA1 with S100A11.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Mus musculus (Mouse,小鼠) 应用(仅供研究使用,不用于临床诊断!)
RPE787Mu01 膜联蛋白A1(ANXA1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPE787Mu02 膜联蛋白A1(ANXA1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APE787Mu01 膜联蛋白A1(ANXA1)活性蛋白 Cell culture; Activity Assays.
PAE787Mu01 膜联蛋白A1(ANXA1)多克隆抗体 WB; IHC; ICC; IP.
PAE787Mu02 膜联蛋白A1(ANXA1)多克隆抗体 WB; IHC; ICC; IP.
LAE787Mu71 膜联蛋白A1(ANXA1)多克隆抗体(生物素标记) WB; IHC; ICC.
SEE787Mu 膜联蛋白A1(ANXA1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
KSE787Mu01 膜联蛋白A1(ANXA1)检测试剂盒DIY材料(酶联免疫吸附试验法) 自己动手制作ELISA试剂盒的主要材料

参考文献

杂志 参考文献
Plos one Dysregulation of Anti-Inflammatory Annexin A1 Expression in Progressive Crohns Disease [PubMed: PMC3794972]
African Journals Online Modulatory Effect of Phytoestrogens and Curcumin on Induction of Annexin 1 in Human Peripheral Blood Mononuclear Cells and their Inhibitory Effect on Secretory Phospholipase A2 [Ajol: Source]
Antimicrobial Agents and Chemotherapy Host Biomarkers of Invasive Pulmonary Aspergillosis to Monitor Therapeutic Response [Asm: Source]
J Proteomics Characterization of secretomes from a human blood brain barrier endothelial cells in-vitro model after ischemia by stable isotope labeling with aminoacids in cell culture (SILAC) [PubMed: 26718731]
Diabetes Annexin A1 Is a Key Modulator of Mesenchymal Stromal Cell–Mediated Improvements in Islet Function [PubMed: 26470781]
PLoS One Annexin A1 Is Increased in the Plasma of Preeclamptic Women [PubMed: 26398190]
中国现代医学杂志 China Journal of Modern Medicine 膜联蛋白A1 和CA19-9 在胆管癌中的表达及其临床意义 [article:8686]
arteriosclerosis thrombosis and vascular biology Protective Aptitude of Annexin A1 in Arterial Neointima Formation in Atherosclerosis-Prone Mice-Brief Report. [pubmed:28062503]
Clinical Science Mesenchymal stromal cells improve human islet function through released products and extracellular matrix. [pubmed:29101297]
Biomedicine & Pharmacotherapy Proresolving protein Annexin A1: The role in type 2 diabetes mellitus and obesity [Pubmed:29677533]
 International Journal of Chronic Obstructive Pulmonary Disease Annexin A1 is elevated in patients with COPD and affects lung fibroblast function [Pubmed:29440885]
Medical Mycology Applying host disease status biomarkers to therapeutic response monitoring in invasive aspergillosis patients [Pubmed:29370415]
DEVELOPMENTAL NEUROPSYCHOLOGY EFFECTS OF MULTICOMPONENT EXERCISE ON PROGRESSION OF BRAIN ATROPHY AND COGNITION IN AMNESTIC MILD COGNITIVE IMPAIRMENT … [pdf:3395]
Multiple sclerosis and related disorders Resolution of inflammation in neuromyelitis optica spectrum disorders [Pubmed: 30300851]
Acta Pharmaceutica Effects of annexin A1 on apoptosis and cell cycle arrest in human leukemic cell lines []
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