膜联蛋白A1(ANXA1)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Annexin A1 (ANXA1)

ANX-A1; ANX1; LPC1; Lipocortin I; Chromobindin-9; Calpactin II; Phospholipase A2 inhibitory protein

  • 膜联蛋白A1(ANXA1)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 膜联蛋白A1(ANXA1)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对膜联蛋白A1(ANXA1)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种:Mus musculus (Mouse,小鼠) 应用(仅供研究使用,不用于临床诊断!)
RPE787Mu01 膜联蛋白A1(ANXA1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPE787Mu02 膜联蛋白A1(ANXA1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APE787Mu01 膜联蛋白A1(ANXA1)活性蛋白 Cell culture; Activity Assays.
PAE787Mu01 膜联蛋白A1(ANXA1)多克隆抗体 WB; IHC; ICC; IP.
PAE787Mu02 膜联蛋白A1(ANXA1)多克隆抗体 WB; IHC; ICC; IP.
LAE787Mu71 膜联蛋白A1(ANXA1)多克隆抗体(生物素标记) WB; IHC; ICC.
SEE787Mu 膜联蛋白A1(ANXA1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
KSE787Mu01 膜联蛋白A1(ANXA1)检测试剂盒DIY材料(酶联免疫吸附试验法) 自己动手制作ELISA试剂盒的主要材料

参考文献

杂志 参考文献
Plos one Dysregulation of Anti-Inflammatory Annexin A1 Expression in Progressive Crohns Disease [PubMed: PMC3794972]
African Journals Online Modulatory Effect of Phytoestrogens and Curcumin on Induction of Annexin 1 in Human Peripheral Blood Mononuclear Cells and their Inhibitory Effect on Secretory Phospholipase A2 [Ajol: Source]
Antimicrobial Agents and Chemotherapy Host Biomarkers of Invasive Pulmonary Aspergillosis to Monitor Therapeutic Response [Asm: Source]
J Proteomics Characterization of secretomes from a human blood brain barrier endothelial cells in-vitro model after ischemia by stable isotope labeling with aminoacids in cell culture (SILAC) [PubMed: 26718731]
Diabetes Annexin A1 Is a Key Modulator of Mesenchymal Stromal Cell–Mediated Improvements in Islet Function [PubMed: 26470781]
PLoS One Annexin A1 Is Increased in the Plasma of Preeclamptic Women [PubMed: 26398190]
中国现代医学杂志 China Journal of Modern Medicine 膜联蛋白A1 和CA19-9 在胆管癌中的表达及其临床意义 [article:8686]
arteriosclerosis thrombosis and vascular biology Protective Aptitude of Annexin A1 in Arterial Neointima Formation in Atherosclerosis-Prone Mice-Brief Report. [pubmed:28062503]
Clinical Science Mesenchymal stromal cells improve human islet function through released products and extracellular matrix. [pubmed:29101297]
Biomedicine & Pharmacotherapy Proresolving protein Annexin A1: The role in type 2 diabetes mellitus and obesity [Pubmed:29677533]
 International Journal of Chronic Obstructive Pulmonary Disease Annexin A1 is elevated in patients with COPD and affects lung fibroblast function [Pubmed:29440885]
Medical Mycology Applying host disease status biomarkers to therapeutic response monitoring in invasive aspergillosis patients [Pubmed:29370415]
DEVELOPMENTAL NEUROPSYCHOLOGY EFFECTS OF MULTICOMPONENT EXERCISE ON PROGRESSION OF BRAIN ATROPHY AND COGNITION IN AMNESTIC MILD COGNITIVE IMPAIRMENT … [pdf:3395]
Multiple sclerosis and related disorders Resolution of inflammation in neuromyelitis optica spectrum disorders [Pubmed: 30300851]
Acta Pharmaceutica Effects of annexin A1 on apoptosis and cell cycle arrest in human leukemic cell lines []
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