Active Collagen Type VI Alpha 1 (COL6a1)
COL6-A1; OPLL; Collagen Alpha-1(VI)chain
- 物种Mus musculus (Mouse，小鼠) 相同的名称，不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 纯度> 95%
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1858 ¥ 4644 ¥ 9288 ¥ 27864 ¥ 69660
- Figure. SDS-PAGE
- Figure. Western Blot
- 通过ISO 9001、ISO 13485质量体系认证
Figure. The binding activity of COL6a1 with PDGFA.
Collagen alpha-1(VI) chain (COL6a1) is a protein that in humans, the collagens are a superfamily of proteins that play a role in maintaining the integrity of various tissues. Collagens are extracellular matrix proteins and have a triple-helical domain as their common structural element. Besides, Platelet-derived growth factor subunit A (PDGFA) has been identified as an interactor of COL6A1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human COL6a1 and recombinant human PDGFA. Briefly, COL6a1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to PDGFA-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-COL6a1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of COL6a1 and PDGFA was shown in Figure 1, and this effect was in a dose dependent manner.
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
|编号||适用物种：Mus musculus (Mouse，小鼠)||应用(仅供研究使用，不用于临床诊断！)|
|APC150Mu03||Ⅵ型胶原α1(COL6a1)活性蛋白||Cell culture; Activity Assays.|
|RPC150Mu02||Ⅵ型胶原α1(COL6a1)重组蛋白||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPC150Mu03||Ⅵ型胶原α1(COL6a1)重组蛋白||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPC150Mu01||Ⅵ型胶原α1(COL6a1)重组蛋白||Positive Control; Immunogen; SDS-PAGE; WB.|
|APC150Mu02||Ⅵ型胶原α1(COL6a1)活性蛋白||Cell culture; Activity Assays.|
|PAC150Mu01||Ⅵ型胶原α1(COL6a1)多克隆抗体||WB; IHC; ICC; IP.|
|PAC150Mu03||Ⅵ型胶原α1(COL6a1)多克隆抗体||WB; IHC; ICC; IP.|
|PAC150Mu02||Ⅵ型胶原α1(COL6a1)多克隆抗体||WB; IHC; ICC; IP.|
|LAC150Mu71||Ⅵ型胶原α1(COL6a1)多克隆抗体(生物素标记)||WB; IHC; ICC.|
|SEC150Mu||Ⅵ型胶原α1(COL6a1)检测试剂盒(酶联免疫吸附试验法)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|Molecular & Cellular Proteomics||Integrated Proteomic Profiling of Cell Line Conditioned Media and Pancreatic Juice for the Identification of Pancreatic Cancer Biomarkers [PubMed: PMC3205865]|