Ⅵ型胶原α1(COL6a1)活性蛋白

Active Collagen Type VI Alpha 1 (COL6a1)

COL6-A1; OPLL; Collagen Alpha-1(VI)chain

  • Ⅵ型胶原α1(COL6a1)活性蛋白 产品包装(模拟)
  • Ⅵ型胶原α1(COL6a1)活性蛋白 产品包装(模拟)
  • APC150Mu02.jpg Figure. SDS-PAGE
  • Ⅵ型胶原α1(COL6a1)活性蛋白 Figure. Western Blot
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Figure. The binding activity of COL6a1 with PDGFA.
Collagen alpha-1(VI) chain (COL6a1) is a protein that in humans, the collagens are a superfamily of proteins that play a role in maintaining the integrity of various tissues. Collagens are extracellular matrix proteins and have a triple-helical domain as their common structural element. Besides, Platelet-derived growth factor subunit A (PDGFA) has been identified as an interactor of COL6A1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human COL6a1 and recombinant human PDGFA. Briefly, COL6a1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to PDGFA-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-COL6a1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of COL6a1 and PDGFA was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Mus musculus (Mouse,小鼠) 应用(仅供研究使用,不用于临床诊断!)
RPC150Mu02 Ⅵ型胶原α1(COL6a1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPC150Mu03 Ⅵ型胶原α1(COL6a1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPC150Mu01 Ⅵ型胶原α1(COL6a1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APC150Mu02 Ⅵ型胶原α1(COL6a1)活性蛋白 Cell culture; Activity Assays.
PAC150Mu01 Ⅵ型胶原α1(COL6a1)多克隆抗体 WB; IHC; ICC; IP.
PAC150Mu03 Ⅵ型胶原α1(COL6a1)多克隆抗体 WB; IHC; ICC; IP.
PAC150Mu02 Ⅵ型胶原α1(COL6a1)多克隆抗体 WB; IHC; ICC; IP.
LAC150Mu71 Ⅵ型胶原α1(COL6a1)多克隆抗体(生物素标记) WB; IHC; ICC.
SEC150Mu Ⅵ型胶原α1(COL6a1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.

参考文献

杂志 参考文献
Molecular & Cellular Proteomics Integrated Proteomic Profiling of Cell Line Conditioned Media and Pancreatic Juice for the Identification of Pancreatic Cancer Biomarkers [PubMed: PMC3205865]
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