胰岛素样生长因子1(IGF1)活性蛋白

Active Insulin Like Growth Factor 1 (IGF1)

IGFI; IGF1A; IBP1; MGF; Somatomedin C; Mechano Growth Gactor

  • 胰岛素样生长因子1(IGF1)活性蛋白 产品包装(模拟)
  • 胰岛素样生长因子1(IGF1)活性蛋白 产品包装(模拟)
  • 胰岛素样生长因子1(IGF1)活性蛋白 Figure. Gene Sequencing (Extract)
  • APA050Hu01.png Figure. SDS-PAGE
  • 胰岛素样生长因子1(IGF1)活性蛋白 Figure. Western Blot
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Figure. The binding activity of IGF1 with IGFBP4.
Insulin-like growth factor 1 (IGF1), also called somatomedin C is a hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. A synthetic analog of IGF1, mecasermin, is used for the treatment of growth failure. IGF1 consists of 70 amino acids in a single chain with three intramolecular disulfide bridges. IGF1 has a molecular weight of 7,649 Daltons. Besides, Insulin Like Growth Factor Binding Protein 4 (IGFBP4) has been identified as an interactor of IGF1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IGF1 and recombinant human IGFBP4. Briefly, IGF1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100µL were then transferred to IGFBP4-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-VDR pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IGF1 and IGFBP4 was shown in Figure 1, and this effect was in a dose dependent manner.

To measure the effect of IGF1 on cell proliferation, breast cancer MCF-7 cells were seeded into triplicate wells of 96-well plates at a density of 5,000 cells/well and allowed to attach, replaced with serum-free overnight, then the medium was replaced with 1% serum standard DMEM prior to the addition of various concentrations of recombinant human IGF1. After incubated for 96h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37℃. Proliferation of MCF-7 cells after incubation with IGF1 for 72h observed by inverted microscope was shown in Figure 2. Cell viability was assessed by CCK-8 assay after incubation with recombinant IGF1 for 72h. The result was shown in Figure 3. It was obvious that IGF1 significantly increased cell viability of MCF-7 cells.
(A) MCF-7 cells cultured in DMEM, stimulated with 10ng/mL IGF1 for 72h;
(B) Unstimulated MCF7 cells cultured in DMEM for 72h.
Figure. Cell proliferation of MCF-7 cells after stimulated with IGF1.

Figure. Cell proliferation of MCF-7 cells after stimulated with IGF1.

用法

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
EPA050Hu61 胰岛素样生长因子1(IGF1)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA050Hu01 胰岛素样生长因子1(IGF1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA050Hu61 胰岛素样生长因子1(IGF1)活性蛋白 Cell culture; Activity Assays.
APA050Hu01 胰岛素样生长因子1(IGF1)活性蛋白 Cell culture; Activity Assays.
CPA050Hu21 胰岛素样生长因子1(IGF1)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
PAA050Hu08 胰岛素样生长因子1(IGF1)多克隆抗体 WB; IHC; ICC; IP.
PAA050Hu01 胰岛素样生长因子1(IGF1)多克隆抗体 WB; IHC; ICC; IP.
PAA050Hu06 胰岛素样生长因子1(IGF1)多克隆抗体 WB; IHC; ICC; IP.
LAA050Hu71 胰岛素样生长因子1(IGF1)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA050Hu22 胰岛素样生长因子1(IGF1)单克隆抗体 WB; IHC; ICC; IP.
MAA050Hu21 胰岛素样生长因子1(IGF1)单克隆抗体 WB; IHC; ICC; IP.
MAA050Hu25 胰岛素样生长因子1(IGF1)单克隆抗体 WB; IHC; ICC; IP.
MAA050Hu23 胰岛素样生长因子1(IGF1)单克隆抗体 WB; IHC; ICC; IP.
LAA050Hu72 胰岛素样生长因子1(IGF1)单克隆抗体(生物素标记) WB; IHC; ICC.
SEA050Hu 胰岛素样生长因子1(IGF1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
HEA050Hu 胰岛素样生长因子1(IGF1)检测试剂盒(酶联免疫吸附试验法,高敏型) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA050Hu 胰岛素样生长因子1(IGF1)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
PSA050Hu01 胰岛素样生长因子1(IGF1)抗体对 ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA050Hu01 胰岛素样生长因子1(IGF1)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

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