胰岛素样生长因子1(IGF1)活性蛋白

Active Insulin Like Growth Factor 1 (IGF1)

IGFI; IGF1A; IBP1; MGF; Somatomedin C; Mechano Growth Gactor

  • 胰岛素样生长因子1(IGF1)活性蛋白 产品包装(模拟)
  • 胰岛素样生长因子1(IGF1)活性蛋白 产品包装(模拟)
  • 胰岛素样生长因子1(IGF1)活性蛋白 Gene sequencing
  • APA050Hu61.jpg SDS-PAGE图
  • 胰岛素样生长因子1(IGF1)活性蛋白 Figure. SDS-PAGE; Sample: Active recombinant IGF1, Human.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Insulin-like growth factor I (IGF1), is a hormone similar in molecular structure to insulin but have a much higher growth-promoting activity, it belongs to a family of proteins involved in mediating growth and development. It is reported that IGF1 induces the proliferation, migration, differentiation of a large types of cells including the MCF-7 breast cancer cell line. To test the effect of growth factors on proliferation, MCF-7 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of IGF-1. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37°C.
Cell proliferation of MCF-7 cells after incubation with IGF1 for 72h observed by inverted microscope was shown in Figure 1.

The dose-effect curve of IGF1 was shown in Figure2. It was obvious that IGF1 significantly promoted cell proliferation of MCF-7 cells. The ED50 for this effect is typically 8.66~17.19 ng/mL.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
EPA050Hu61 胰岛素样生长因子1(IGF1)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA050Hu01 胰岛素样生长因子1(IGF1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA050Hu61 胰岛素样生长因子1(IGF1)活性蛋白 Cell culture; Activity Assays.
APA050Hu01 胰岛素样生长因子1(IGF1)活性蛋白 Cell culture; Activity Assays.
CPA050Hu21 胰岛素样生长因子1(IGF1)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
PAA050Hu08 胰岛素样生长因子1(IGF1)多克隆抗体 WB; IHC; ICC; IP.
PAA050Hu01 胰岛素样生长因子1(IGF1)多克隆抗体 WB; IHC; ICC; IP.
PAA050Hu06 胰岛素样生长因子1(IGF1)多克隆抗体 WB; IHC; ICC; IP.
LAA050Hu71 胰岛素样生长因子1(IGF1)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA050Hu22 胰岛素样生长因子1(IGF1)单克隆抗体 WB; IHC; ICC; IP.
MAA050Hu21 胰岛素样生长因子1(IGF1)单克隆抗体 WB; IHC; ICC; IP.
LAA050Hu72 胰岛素样生长因子1(IGF1)单克隆抗体(生物素标记) WB; IHC; ICC.
SEA050Hu 胰岛素样生长因子1(IGF1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
HEA050Hu 胰岛素样生长因子1(IGF1)检测试剂盒(酶联免疫吸附试验法,高敏型) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA050Hu 胰岛素样生长因子1(IGF1)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
PSA050Hu01 胰岛素样生长因子1(IGF1)抗体对 ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA050Hu01 胰岛素样生长因子1(IGF1)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Cellular & Molecular Biology Letters A microarray gene analysis of peripheral whole blood in normal adult male rats after long-term GH gene therapy [PubMed: 20119855]
Molecular Biology Reports Characterization of the specific and sustained GH1 expression induced by rAAV2/1 in normal adult male rats [PubMed: 20204528]
Regulatory Peptides The vagus nerve as a regulator of growth hormone secretion [PubMed: 21056594]
Vascular Cell Insulin-Like Growth Factor-1 (IGF-1) Reduces Ischemic Changes and Increases Circulating Angiogenic Factors in Experimentally - Induced Myocardial Infarction in Rats [PubMed: 21651821]
27 Physical activity attenuates intermittent hypoxia-induced spatial learning deficits and oxidative stress. [PubMed: 20224062]
Journal of Pediatric Surgery The influence of nutrients, biliary-pancreatic secretions, and systemic trophic hormones on intestinal adaptation in a Roux-en-Y bypass model [PubMed: 20438940]
The Veterinary Journal Serum insulin-like growth factor type 1 concentrations in healthy dogs and dogs with spontaneous primary hypothyroidism [PubMed: 21546289]
Developmental Biology Effects of monochromatic light on developmental changes in satellite cell population of pectoral muscle in broilers during early posthatch period [Wiley: source]
Journal of Neuroendocrinology Interleukin-6 Regulates the Expression of Hypothalamic Neuropeptides Involved in Body Weight in a Gender-Dependent Way [Wiley: source]
International Journal of Molecular Sciences Insulin Like Growth Factor-1 (IGF-1) Causes Overproduction of IL-8, an Angiogenic Cytokine and Stimulates Neovascularization in Isoproterenol-Induced Myocardial Infarction in Rats [Mdpi: 8562]
Pharmacology Protective Effect of Amlodipine on Rat Bone Tissue after Orchidectomy [Karger: 335491]
Bulletin of the Veterinary Institute in Pulawy Maternal Glucocorticoid Treatment as a Model for Examining Foetal Gender-Specific Effects on the Bone Metabolism of Neonatal Piglets [Degruyter: Source]
BMC cell biology BMSCs reduce rat granulosa cell apoptosis induced by cisplatin and perimenopause [PubMed: PMC3640998]
Journal of Veterinary Science Evaluation of IGF-I levels and serum protein profiles of diabetic cats and dogs [PubMed: PMC3232391]
PLoS ONE A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches [Plosone: Source]
Pesquisa Veterinária Brasileira Porcine follicular fluid concentration of free insulin-like growth factor-I collected from different diameter ovarian follicles [Scielo: Source]
international Journal of food and nutritional sciences EVALUATION OF PHYSICO-CHEMICAL PROPERTIES OF COLOSTRUM SUPPLEMENTED DAHI [Ijfans: Source]
Theriogenology Role of insulin-like growth factor-I and follicular fluid from ovarian follicles with different diameters on porcine oocyte maturation and fertilization in vitro [ScienceDirect: S0093691X13001659]
Biosensors and Bioelectronics Electrochemical immunosensor for the determination of insulin-like growth factor-1 using electrodes modified with carbon nanotubes–poly(pyrrole propionic acid) hybrids [ScienceDirect: S0956566313005629]
Electromagnetic Biology and Medicine The effects of long-term exposure to a 2450 MHz electromagnetic field on growth and pubertal development in female Wistar rats [Informahealthcare: Source]
Nutrition and Cancer A Pilot Study Comparing the Effect of Flaxseed, Aromatase Inhibitor, and the Combination on Breast Tumor Biomarkers [Tandfonline: Source]
Bases de Dados da Pesquisa Agropecuária CONCENTRACOES SéRICAS DE INSULINA NO PóS-PARTO RECENTE DE VACAS DE CORTE DE DIFERENTES GENóTIPOS CRIADAS NO SUL DO BRASIL [Embrapa: Source]
International Journal of Endocrinology Negative Energy Balance Induced by Paradoxical Sleep Deprivation Causes Multicompartmental Changes in Adipose Tissue and Skeletal Muscle [Cepebr:Source]
J Neurol Sci. The effect of topiramate and lamotrigine on rat bone mass, structure and metabolism. [Pubmed:24629477]
Reproductive Sciences Effects of Resveratrol on Ovarian Morphology, Plasma Anti-Mullerian Hormone, IGF-1 Levels, and Oxidative Stress Parameters in a Rat Model of Polycystic Ovary Syndrome [Pubmed:25667201]
Int J Clin Exp Med Effects of sericin on the testicular growth hormone/insulin-like growth factor-1 axis in a rat model of type 2 diabetes [PubMed: 26379831]
Int J Clin Exp Med. The effects of self-assembling peptide RADA16 hydrogel on malignant phenotype of human hepatocellular carcinoma cell [PubMed: 26628972]
Animal and Biomedical Sciences The use of herb mix and lucerne to increase growth rates of dairy heifers [System: Files]
Neonatal Biology Maternal Nutrition with Β-Hydroxy-Β-Methylbutyrate as Strong Determinants of the Development of Newborn Offspring in Pigs [Journals: 1000183]
Dig Dis Sci The Protective Effects of Exclusive Enteral Nutrition Formulas on Growth Factor Expression and the Proximal Tibial Epiphyseal Growth Plate in a TNBS-Induced IBD Rat Model [PubMed: 25708898]
Tumor Biology Hypoxia-induced secretion of IL-12 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphoma [PubMed: 26695151]
Forage Res RELATIONSHIP OF RESIDUAL FEED INTAKE WITH BLOOD METABOLITES AND HORMONES IN SAHIWAL FEMALE CALVES [Wp-Content: Uploads]
S Caracteriza [Teses: Disponiveis]
Photochemistry AND Photobiology Melatonin Mediates Monochromatic Light‐induced Insulin‐like Growth Factor 1 Secretion of Chick Liver: Involvement of Membrane Receptors [Pubmed:27128575]
Pediatric Pulmonology Protective effect of vitamin D against hyperoxia‐induced lung injury in newborn rats [Pubmed:27291304]
Hippocampus A single bout of resistance exercise improves memory consolidation and increases the expression of synaptic proteins in the hippocampus [Pubmed:27008926]
Neurotoxicity Research Cigarette Smoke Delays Regeneration of the Olfactory Epithelium in Mice [Pubmed:27003941]
ANNALS OF ANIMAL SCIENCE Comparison of the effect of a standard inclusion level of inorganic zinc to organic form at lowered level on bone development in growing male Ross broiler chickens [journalprintahead:87]
Annals of Animal Science Comparison of the Effect of a Standard Inclusion Level of Inorganic Zinc to Organic Form at Lowered Level on Bone Development in Growing Male Ross Broiler Chickens [view:87]
African Health Sciences Effects of acute exercise on salivary free insulin-like growth factor 1 and interleukin 10 in sportsmen. [pubmed:27605972]
PLoS One. Paradoxical Sleep Deprivation Causes Cardiac Dysfunction and the Impairment Is Attenuated by Resistance Training. [pubmed:27880816]
international journal of molecular sciences Expression of Iron-Related Proteins Differentiate Non-Cancerous and Cancerous Breast Tumors. [pubmed:28216608]
Biochemistry and Cell Biology Chronic intermittent hypoxia disturbs insulin secretion and causes pancreatic injury via theMAPK signaling pathway. [pubmed:28177762]
Neurochemical Research Resistance Exercise Reduces Seizure Occurrence, Attenuates Memory Deficits and Restores BDNF Signaling in Rats with Chronic Epilepsy [article:10.1007]
Journal of Agricultural Biotechnology GH蛋白诱导鸡原代成肌细胞表达IGF1条件优化 [10697]
Trop Anim Health Prod Assessment of feeding varying levels of Metabolizable energy and protein on performance of transition Murrah buffaloes [pubmed:28785907]
Molecular Medicine Reports Mesenchymal stem cells promote endothelial progenitor cell proliferation by secreting insulin‑like growth factor‑1 [pubmed:28627605]
Neurochem Res Resistance Exercise Reduces Seizure Occurrence, Attenuates Memory Deficits and Restores BDNF Signaling in Rats with Chronic Epilepsy [pubmed:28078614]
Biological Trace Element Research Effect of Dietary Phytase Supplementation on Bone and Hyaline Cartilage Development of Broilers Fed with Organically Complexed Copper in a Cu-Deficient Diet [article:10.1007]
Revista Brasileira de Ciência Avícola Effect of Zinc Level and Source (Zinc Oxide Vs. Zinc Glycine) on Bone Mechanical and Geometric Parameters, and Histomorphology in Male Ross 308 Broiler Chicken [10.159:1806-9061-2016-0285]
International Journal of Chemical Studies Studies on quality attributes of skimmed colostrum powder [P-ISSN: 2349–8528]
Stem Cell Research & Therapy Effects of low-intensity pulsed ultrasound (LIPUS)-pretreated human amnion-derived mesenchymal stem cell (hAD-MSC) transplantation on primary ovarian insufficiency in rats [10.1186/s13287-017-0739-3]
PLOS one A metabolite of leucine (β-hydroxy-β-methylbutyrate) given to sows during pregnancy alters bone development of their newborn offspring by hormonal modulation [10.1371/journal.pone.0179693]
Journal of Controlled Release A DPP-IV-resistant glucagon-like peptide-2 dimer with enhanced activity against radiation-induced intestinal injury [pubmed:28522195]
Obesity Surgery Effects of Bariatric Surgery on Change of Brown Adipocyte Tissue and Energy Metabolism in Obese Mice [pubmed:28853013]
Journal of Nutritional Sciences Effect of dietary protein intake on the body composition and metabolic parameters of neutered dogs [pubmed:29152244]
Inflammation Involvements of γδT Lymphocytes in Acute and Chronic Skin Wound Repair. [pubmed:28540539]
Frontiers in Molecular Neuroscience Rosiglitazone Exerts an Anti-depressive Effect in Unpredictable Chronic Mild-Stress-Induced Depressive Mice by Maintaining Essential Neuron Autophagy and Inhibiting Excessive Astrocytic Apoptosis. [pubmed:28959186]
Journal of Photochemistry and Photobiology Various LED Wavelengths Affected Myofiber Development and Satellite Cell Proliferation of Chick Embryos via the IGF-1 Signaling Pathway. [pubmed:28708285]
留言咨询