肝素酶(HPA)多克隆抗体

Polyclonal Antibody to Heparanase (HPA)

HPSE; HPR1; HPSE1; HSE1; Endo-glucoronidase;

  • 肝素酶(HPA)多克隆抗体 产品包装(模拟)
  • 肝素酶(HPA)多克隆抗体 产品包装(模拟)
  • 肝素酶(HPA)多克隆抗体 Used in DAB staining on fromalin fixed paraffin-embedded Spleen tissue
  • PAA711Hu02.jpg Figure. Western Blot; Sample: Recombinant HPA, Human.
  • 肝素酶(HPA)多克隆抗体 Figure. Western Blot; Sample: Lane1: Human Hela Cells; Lane2: Human HepG2 Cells; Lane3: Human Liver Tissue.
  • 肝素酶(HPA)多克隆抗体 Knockout Varification:
    Lane 1: Wild-type HepG2 cell lysate;
    Lane 2: HPA knockout HepG2 cell lysate;
    Predicted MW: 43,53,55,61kd
    Observed MW: 68kd
    Primary Ab: 1µg/ml Rabbit Anti-Human HPA Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 肝素酶(HPA)多克隆抗体 HepG2 human hepatocellular carcinoma cell line was stained with rabbit Anti-human HPA(HPSE) Polyclonal Antibody (Catalog # PAA711Hu02, filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled green histogram), followed by APC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb15). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

该抗体是针对HPA的兔多克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别HPA。

用法

Western blotting: 0.5-2µg/mL
Immunocytochemistry in formalin fixed cells: 5-20µg/mL
Immunohistochemistry in formalin fixed frozen section: 5-20µg/mL
Immunohistochemistry in paraffin section: 5-20µg/mL
Optimal working dilutions must be determined by end user.

储存

经常使用则4°C保存。-20°C保存不超过一年。避免反复冻融。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPA711Hu04 肝素酶(HPA)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA711Hu02 肝素酶(HPA)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA711Hu01 肝素酶(HPA)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA711Hu03 肝素酶(HPA)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
CPA711Hu21 肝素酶(HPA)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
PAA711Hu04 肝素酶(HPA)多克隆抗体 WB; IHC; ICC; IP.
PAA711Hu02 肝素酶(HPA)多克隆抗体 WB; IHC; ICC; IP.
PAA711Hu01 肝素酶(HPA)多克隆抗体 WB; IHC; ICC; IP.
PAA711Hu03 肝素酶(HPA)多克隆抗体 WB; IHC; ICC; IP.
LAA711Hu71 肝素酶(HPA)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA711Hu22 肝素酶(HPA)单克隆抗体 WB; IHC; ICC; IP.
MAA711Hu21 肝素酶(HPA)单克隆抗体 WB; IHC; ICC; IP.
SEA711Hu 肝素酶(HPA)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
HEA711Hu 肝素酶(HPA)检测试剂盒(酶联免疫吸附试验法,高敏型) Enzyme-linked immunosorbent assay for Antigen Detection.
KSA711Hu01 肝素酶(HPA)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Plos one PG545, a Heparan Sulfate Mimetic, Reduces Heparanase Expression In Vivo, Blocks Spontaneous Metastases and Enhances Overall Survival in the 4T1 Breast Carcinoma Model [Plosone: Source]
Journal of Medical Science Assessment of heparanase and heparin-binding growth and angiogenesis factors in the uterine cavity ? uid in women with impaired reproduction [Nowinylekarskie:Source]
Int J Clin Exp Pathol Unfractionated heparin attenuates intestinal injury in mouse model of sepsis by inhibiting heparanase [PubMed: 26191183]
Tumour Biology PI-88 inhibits postoperative recurrence of hepatocellular carcinoma via disrupting the surge of heparanase after liver resection [PubMed: 26415733]
Journal of Medical Science Assessment of heparanase and heparin-binding growth and angiogenesis factors in the uterine cavity fluid in women with impaired reproduction [article:84]
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