肝素酶(HPSE)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Heparanase (HPSE)
HPA; HPR1; HPSE1; HSE1; Endo-glucoronidase;
- 编号KSA711Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 试剂内容 包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围31.2-2000pg/mL
- 可适灵敏度12.3pg/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载 英文说明书 中文说明书
- 规格 96T*596T*10 96T*20 96T*50 96T*100
- 价格 ¥ 6804 ¥ 11340 ¥ 20412 ¥ 39690 ¥ 68040
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
-
产品包装(模拟)
-
产品包装(模拟)
-
通过ISO 9001、ISO 13485质量体系认证
特异性
试剂盒中的抗体对肝素酶(HPA)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA711Hu04 | 肝素酶(HPSE)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA711Hu02 | 肝素酶(HPSE)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA711Hu04 | 肝素酶(HPSE)活性蛋白 | Cell culture; Activity Assays. |
| APA711Hu01 | 肝素酶(HPSE)活性蛋白 | Cell culture; Activity Assays. |
| EPA711Hu61 | 肝素酶(HPSE)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA711Hu61 | 肝素酶(HPSE)活性蛋白 | Cell culture; Activity Assays. |
| RPA711Hu01 | 肝素酶(HPSE)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA711Hu03 | 肝素酶(HPSE)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| CPA711Hu21 | 肝素酶(HPSE)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| PAA711Hu04 | 肝素酶(HPSE)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA711Hu02 | 肝素酶(HPSE)多克隆抗体 | WB,IHC,ICC/IF |
| PAA711Hu01 | 肝素酶(HPSE)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA711Hu03 | 肝素酶(HPSE)多克隆抗体 | WB; IHC; ICC; IP. |
| FAA711Hu01 | 抗肝素酶(HPSE)多克隆抗体 | Flow cytometry. |
| LAA711Hu71 | 肝素酶(HPSE)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAA711Hu23 | 肝素酶(HPSE)单克隆抗体 | WB |
| MAA711Hu22 | 肝素酶(HPSE)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA711Hu21 | 肝素酶(HPSE)单克隆抗体 | WB,ICC/IF |
| SEA711Hu | 肝素酶(HPSE)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEA711Hu | 肝素酶(HPSE)检测试剂盒(酶联免疫吸附试验法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA711Hu | 肝素酶(HPSE)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA711Hu01 | 肝素酶(HPSE)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Plos one | PG545, a Heparan Sulfate Mimetic, Reduces Heparanase Expression In Vivo, Blocks Spontaneous Metastases and Enhances Overall Survival in the 4T1 Breast Carcinoma Model [Plosone: Source] |
| Journal of Medical Science | Assessment of heparanase and heparin-binding growth and angiogenesis factors in the uterine cavity ? uid in women with impaired reproduction [Nowinylekarskie:Source] |
| Int J Clin Exp Pathol | Unfractionated heparin attenuates intestinal injury in mouse model of sepsis by inhibiting heparanase [PubMed: 26191183] |
| Tumour Biology | PI-88 inhibits postoperative recurrence of hepatocellular carcinoma via disrupting the surge of heparanase after liver resection [PubMed: 26415733] |
| Journal of Medical Science | Assessment of heparanase and heparin-binding growth and angiogenesis factors in the uterine cavity fluid in women with impaired reproduction [article:84] |
| Renal Failure | Plasma heparanase is associated with blood glucose levels but not urinary microalbumin excretion in type 2 diabetic nephropathy at the early stage [pubmed:28994624] |
留言咨询
