晚期糖基化终末产物(AGE)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Advanced Glycation End Product (AGE)

AGEs; Advanced Glycation End Products

  • 晚期糖基化终末产物(AGE)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 晚期糖基化终末产物(AGE)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对晚期糖基化终末产物(AGE)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种:Pan-species (General,通用) 应用(仅供研究使用,不用于临床诊断!)
NPB353Ge01 晚期糖基化终末产物(AGE)天然蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APB353Ge01 晚期糖基化终末产物(AGE)活性蛋白 Cell culture; Activity Assays.
PAB353Ge01 晚期糖基化终末产物(AGE)多克隆抗体 WB; IHC; ICC; IP.
LAB353Ge71 晚期糖基化终末产物(AGE)多克隆抗体(生物素标记) WB; IHC; ICC.
LAB353Ge81 晚期糖基化终末产物(AGE)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
MAB353Ge21 晚期糖基化终末产物(AGE)单克隆抗体 WB; IHC; ICC; IP.
HEB353Ge 晚期糖基化终末产物(AGE)检测试剂盒(酶联免疫吸附试验法,高敏型) Enzyme-linked immunosorbent assay for Antigen Detection.
CEB353Ge 晚期糖基化终末产物(AGE)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
KSB353Ge11 晚期糖基化终末产物(AGE)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Human Immunology Threshold serum concentrations of tumour necrosis factor alpha (TNFα) as a potential marker of the presence of microangiopathy in children and adolescents with type 1 diabetes mellitus (T1DM) [PubMed: S0198885912005642]
Experimental Dermatology A long-standing hyperglycaemic condition impairs skin barrier by accelerating skin ageing process [PubMed: 22017743]
J Hum Nutr Diet. Daily intake of vitamin D- or calcium-vitamin D-fortified Persian yogurt drink (doogh) attenuates diabetes-induced oxidative stress: evidence for antioxidative properties of vitamin D. [Pubmed: 23829785]
Gene Association of RAGE gene polymorphism with circulating AGEs level and paraoxonase activity in relation to macro-vascular complications in Indian type 2 diabetes mellitus patients. [Pubmed: 23721855]
Gynecol Endocrinol. Lipoprotein-associated phospholipase A2 and AGEs are associated with cardiovascular risk factors in women with history of gestational diabetes mellitus. [Pubmed:24397392]
Microvasc Res. Role of advanced glycation end product (AGE)-induced receptor (RAGE) expression in diabetic vascular complications [Pubmed:24984291]
Free Radic Res. Nitroxides prevent protein glycoxidation in vitro. [Pubmed:25363554]
Sleep and Breathing Serum advanced glycation end products are associated with insulin resistance in male nondiabetic patients with obstructive sleep apnea [Pubmed:25566941]
Chem Biol Interact Ascorbic acid and protein glycation [PubMed: 26163454]
Osteoarthritis Cartilage Establishment of a rabbit model to study the influence of advanced glycation end products accumulation on osteoarthritis and the protective effect of pioglitazone [PubMed: 26321377]
journal of human hypertension Relationship between serum levels of endogenous secretory RAGE and blood pressure in male nondiabetic patients with obstructive sleep apnea [PubMed: 25994997]
Redox Biol. Glycation of bovine serum albumin by ascorbate [PubMed: 26202868]
J Oral Pathol Med Impact of morbid obesity and bariatric surgery on antioxidant/oxidant balance of the unstimulated and stimulated human saliva [PubMed: 26608886]
J Agric Food Chem Inhibition of Advanced Glycation Endproduct Formation by Lotus Seedpod Oligomeric Procyanidins through RAGE-MAPK Signaling and NF-κB Activation in High-Fat-Diet Rats. [PubMed: 26207852]
Kineziologija ADVANCED GLYCATION END PRODUCTS'RESPONSE TO RESISTANCE TRAINING IN POSTMENOPAUSAL WOMEN WITH TYPE II DIABETES [140251: ]
Food Funct. Effects of edible bird's nest on hippocampal and cortical neurodegeneration in ovariectomized rats [PubMed: 25920003]
Molecular and Cellular Endocrinology Beneficial effects of urine-derived stem cells on fibrosis and apoptosis of myocardial, glomerular and bladder cells [Pubmed:26952874]
Advances in Experimental Medicine and Biology Effects of S-Nitroso-N-Acetyl-Penicillamine (SNAP) on Inflammation, Lung Tissue Apoptosis and iNOS Activity in a Rabbit Model of Acute Lung Injury [Pubmed:27334732]
Eur J Vasc Endovasc Surg The Receptor for Advanced Glycation End Products (Rage) and Its Ligands in Plasma and InfrainCavia (Guinea pig )inal Bypass Vein [Pubmed:26905625]
molecular and cellular endocrinology Berberine exerts renoprotective effects by regulating the AGEs-RAGE signaling pathway in mesangial cells during diabetic nephropathy. [pubmed:28087385]
cellular physiology and biochemistry Inhibition of Methylglyoxal-Induced AGEs/RAGE Expression Contributes to Dermal Protection by N-Acetyl-L-Cysteine. [pubmed:28214842]
General Physiology and Biophysics Glutathione is the main endogenous inhibitor of protein glycation. [pubmed:28218612]
Osteoarthritis and Cartilage Establishment of a rabbit model to study the influence of advanced glycation end productsaccumulation on osteoarthritis and the protective effect of pioglitazone. [pubmed:26321377]
Scientific Reports Advanced glycation end products promote VEGF expression and thus choroidal neovascularization via Cyr61-PI3K/AKT signaling pathway [pubmed:29097668]
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Lychee seed extract protects against neuronal injury and improves cognitive function in rats with type II diabetes mellitus with cognitive impairment [pubmed:29138799]
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