晚期糖基化终末产物(AGE)等多因子检测试剂盒(流式荧光发光法)
Multiplex Assay Kit for Advanced Glycation End Product (AGE) ,etc. by FLIA (Flow Luminescence Immunoassay)
AGEs; Advanced Glycation End Products
（注：单次混测多因子不超过8个指标 ）
- 编号LMB353Ge
- 物种Pan-species (General，通用) 相同的名称，不同的物种。
- 实验方法竞争抑制
- 反应时长1.5h
- 检测范围7.81-8000ng/mL
- 灵敏度最小可检测剂量小于等于2.603 ng/mL.
- 样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
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产品包装(模拟)
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产品包装(模拟)
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测晚期糖基化终末产物(AGE)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的晚期糖基化终末产物(AGE)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 92-105 | 99 |
| EDTA plasma(n=5) | 85-96 | 93 |
| heparin plasma(n=5) | 94-103 | 98 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的晚期糖基化终末产物(AGE)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中晚期糖基化终末产物(AGE)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 91-98% | 92-105% | 96-103% | 88-99% |
| EDTA plasma(n=5) | 94-101% | 96-104% | 90-101% | 90-97% |
| heparin plasma(n=5) | 90-103% | 78-91% | 82-96% | 85-98% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠、检测溶液A）标准品或样本50μL及磁珠10μL，加检测溶液A50μL，
37°C酶标板振荡器孵育60分钟；
3. 磁吸洗板3次；
4. 加检测溶液B100μL，37°C振动孵育30分钟；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋涡震荡2分钟后读数。
实验原理
将晚期糖基化终末产物(AGE)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠、标记VEGFA，标记的晚期糖基化终末产物(AGE)等多因子检测试剂盒(流式荧光发光法)和未标记的晚期糖基化终末产物(AGE)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体竞争结合，然后将未结合物洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的晚期糖基化终末产物(AGE)等多因子检测试剂盒(流式荧光发光法)呈负相关。
赠品
增值服务
相关产品
| 编号 | 适用物种：Pan-species (General，通用) | 应用(仅供研究使用，不用于临床诊断！) |
| NPB353Ge01 | 晚期糖基化终末产物(AGE)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB353Ge01 | 晚期糖基化终末产物(AGE)活性蛋白 | Cell culture; Activity Assays. |
| PAB353Ge01 | 晚期糖基化终末产物(AGE)多克隆抗体 | WB; IHC; ICC; IP. |
| LAB353Ge71 | 晚期糖基化终末产物(AGE)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| LAB353Ge81 | 晚期糖基化终末产物(AGE)多克隆抗体(异硫氰酸荧光素标记) | IHC; ICC;ELISA |
| MAB353Ge21 | 晚期糖基化终末产物(AGE)单克隆抗体 | WB; IHC; ICC; IP. |
| HEB353Ge | 晚期糖基化终末产物(AGE)检测试剂盒(酶联免疫吸附试验法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEB353Ge | 晚期糖基化终末产物(AGE)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB353Ge | 晚期糖基化终末产物(AGE)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSB353Ge11 | 晚期糖基化终末产物(AGE)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
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| Experimental Dermatology | A long-standing hyperglycaemic condition impairs skin barrier by accelerating skin ageing process [PubMed: 22017743] |
| J Hum Nutr Diet. | Daily intake of vitamin D- or calcium-vitamin D-fortified Persian yogurt drink (doogh) attenuates diabetes-induced oxidative stress: evidence for antioxidative properties of vitamin D. [Pubmed: 23829785] |
| Gene | Association of RAGE gene polymorphism with circulating AGEs level and paraoxonase activity in relation to macro-vascular complications in Indian type 2 diabetes mellitus patients. [Pubmed: 23721855] |
| Gynecol Endocrinol. | Lipoprotein-associated phospholipase A2 and AGEs are associated with cardiovascular risk factors in women with history of gestational diabetes mellitus. [Pubmed:24397392] |
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| Free Radic Res. | Nitroxides prevent protein glycoxidation in vitro. [Pubmed:25363554] |
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| Eur J Vasc Endovasc Surg | The Receptor for Advanced Glycation End Products (Rage) and Its Ligands in Plasma and InfrainCavia (Guinea pig )inal Bypass Vein [Pubmed:26905625] |
| molecular and cellular endocrinology | Berberine exerts renoprotective effects by regulating the AGEs-RAGE signaling pathway in mesangial cells during diabetic nephropathy. [pubmed:28087385] |
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