不对称二甲基精氨酸(ADMA)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Asymmetrical Dimethylarginine (ADMA)

N,N-Dimethylarginine; NG,NG-Dimethylarginine dihydrochloride; Asymmetric Dimethylarginine

  • 不对称二甲基精氨酸(ADMA)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 不对称二甲基精氨酸(ADMA)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对不对称二甲基精氨酸(ADMA)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

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CEB301Ge 不对称二甲基精氨酸(ADMA)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
KSB301Ge11 不对称二甲基精氨酸(ADMA)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
PLoS One. Interactions among Vascular-Tone Modulators Contribute to High Altitude Pulmonary Edema and Augmented Vasoreactivity in Highlanders [PubMed: PMC3439466]
Cytokine Effect of CCL5 on dimethylarginine dimethylaminohydrolase-1 production in vascular smooth muscle cells from spontaneously hypertensive rats. [Pubmed: 23834755]
Neurol Res. Effect of 18β-glycyrrhetinic acid on cerebral vasospasm caused by asymmetric dimethylarginine after experimental subarachnoid hemorrhage in rats. [Pubmed:25475507]
Int J Clin Exp Med. Correlation research on ADMA plasma levels and left ventricular function of peritoneal dialysis patients [Pubmed:25550968]
Clin Respir J Asymmetric dimethylarginine contributes to airway nitric oxide deficiency in patients with COPD [PubMed: 26076870]
Cytokine Dimethylarginine dimethylaminohydrolase-1 mediates inhibitory effect of interleukin-10 on angiotensin II-induced hypertensive effects in vascular smooth muscle cells of spontaneously hypertensive rats [PubMed: 26375520]
Neurological Research Effect of 18β-glycyrrhetinic acid on cerebral vasospasm caused by asymmetric dimethylarginine after experimental subarachnoid hemorrhage in rats [PubMed: 25475507]
Scientific Reports Dimethylarginine Dimethylaminohydrolase 2 (DDAH 2) Gene Polymorphism, Asymmetric Dimethylarginine (ADMA) Concentrations, and Risk of Coronary Artery Disease: A Case-Control Study [pubmed:27677852]
clinica chimica acta Increased serum concentrations of asymmetric dimethylarginine (ADMA) in patients with early-onset coronary artery disease. [pubmed:27884754]
The Journal of Physiology Asymmetric dimethylarginine (ADMA) elevation and arginase up-regulation contribute toendothelial dysfunction related to insulin resistance in rats and morbidly obese humans. [pubmed:26840628]
Angiogenesis Dimethylarginine dimethylaminohydrolase-1 (DDAH1) is frequently upregulated in prostate cancer, and its overexpression conveys tumor growth and angiogenesis by metabolizing asymmetric dimethylarginine (ADMA). [pubmed:29150732]
Scientific Reports A Novel Role of Irbesartan in Gastroprotection against Indomethacin-Induced Gastric Injury in Rats: Targeting DDAH/ADMA and EGFR/ERK Signaling [Pubmed:29523851]
Mechanisms of Ageing and Development Multivessel analysis of progressive vascular aging in the rat: Asynchronous vulnerability among vascular territories [Pubmed:29605639]
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