不对称二甲基精氨酸(ADMA)等多因子检测试剂盒(流式荧光发光法)

Multiplex Assay Kit for Asymmetrical Dimethylarginine (ADMA) ,etc. by FLIA (Flow Luminescence Immunoassay)

N,N-Dimethylarginine; NG,NG-Dimethylarginine dihydrochloride; Asymmetric Dimethylarginine

（注：单次混测多因子不超过8个指标 ）

  • 不对称二甲基精氨酸(ADMA)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • 不对称二甲基精氨酸(ADMA)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测不对称二甲基精氨酸(ADMA)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的不对称二甲基精氨酸(ADMA)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 87-95 90
EDTA plasma(n=5) 79-102 82
heparin plasma(n=5) 83-92 87
sodium citrate plasma(n=5) 83-90 86

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

线性

在定值血清及血浆样本内加入适量的不对称二甲基精氨酸(ADMA)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中不对称二甲基精氨酸(ADMA)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 94-104% 87-101% 84-101% 93-101%
EDTA plasma(n=5) 82-90% 95-102% 93-101% 79-103%
heparin plasma(n=5) 81-90% 81-98% 89-102% 78-93%
sodium citrate plasma(n=5) 89-97% 78-98% 96-104% 93-101%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠、检测溶液A）标准品或样本50μL及磁珠10μL，加检测溶液A50μL，
    37°C酶标板振荡器孵育60分钟；
3. 磁吸洗板3次；
4. 加检测溶液B100μL，37°C振动孵育30分钟；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋涡震荡2分钟后读数。

实验原理

将不对称二甲基精氨酸(ADMA)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠、标记VEGFA，标记的不对称二甲基精氨酸(ADMA)等多因子检测试剂盒(流式荧光发光法)和未标记的不对称二甲基精氨酸(ADMA)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体竞争结合，然后将未结合物洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的不对称二甲基精氨酸(ADMA)等多因子检测试剂盒(流式荧光发光法)呈负相关。

赠品

相关产品

编号 适用物种：Pan-species (General，通用) 应用(仅供研究使用，不用于临床诊断！)
CPB301Ge11 不对称二甲基精氨酸(ADMA)牛血清白蛋白偶联物 Immunogen; SDS-PAGE; WB.
CPB301Ge21 不对称二甲基精氨酸(ADMA)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
PAB301Ge01 不对称二甲基精氨酸(ADMA)多克隆抗体 ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
LAB301Ge71 不对称二甲基精氨酸(ADMA)多克隆抗体(生物素标记) WB; IHC; ICC.
CEB301Ge 不对称二甲基精氨酸(ADMA)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB301Ge 不对称二甲基精氨酸(ADMA)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSB301Ge11 不对称二甲基精氨酸(ADMA)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
PLoS One. Interactions among Vascular-Tone Modulators Contribute to High Altitude Pulmonary Edema and Augmented Vasoreactivity in Highlanders [PubMed: PMC3439466]
Cytokine Effect of CCL5 on dimethylarginine dimethylaminohydrolase-1 production in vascular smooth muscle cells from spontaneously hypertensive rats. [Pubmed: 23834755]
Neurol Res. Effect of 18β-glycyrrhetinic acid on cerebral vasospasm caused by asymmetric dimethylarginine after experimental subarachnoid hemorrhage in rats. [Pubmed:25475507]
Int J Clin Exp Med. Correlation research on ADMA plasma levels and left ventricular function of peritoneal dialysis patients [Pubmed:25550968]
Clin Respir J Asymmetric dimethylarginine contributes to airway nitric oxide deficiency in patients with COPD [PubMed: 26076870]
Cytokine Dimethylarginine dimethylaminohydrolase-1 mediates inhibitory effect of interleukin-10 on angiotensin II-induced hypertensive effects in vascular smooth muscle cells of spontaneously hypertensive rats [PubMed: 26375520]
Neurological Research Effect of 18β-glycyrrhetinic acid on cerebral vasospasm caused by asymmetric dimethylarginine after experimental subarachnoid hemorrhage in rats [PubMed: 25475507]
Scientific Reports Dimethylarginine Dimethylaminohydrolase 2 (DDAH 2) Gene Polymorphism, Asymmetric Dimethylarginine (ADMA) Concentrations, and Risk of Coronary Artery Disease: A Case-Control Study [pubmed:27677852]
clinica chimica acta Increased serum concentrations of asymmetric dimethylarginine (ADMA) in patients with early-onset coronary artery disease. [pubmed:27884754]
The Journal of Physiology Asymmetric dimethylarginine (ADMA) elevation and arginase up-regulation contribute toendothelial dysfunction related to insulin resistance in rats and morbidly obese humans. [pubmed:26840628]
Angiogenesis Dimethylarginine dimethylaminohydrolase-1 (DDAH1) is frequently upregulated in prostate cancer, and its overexpression conveys tumor growth and angiogenesis by metabolizing asymmetric dimethylarginine (ADMA). [pubmed:29150732]
Scientific Reports A Novel Role of Irbesartan in Gastroprotection against Indomethacin-Induced Gastric Injury in Rats: Targeting DDAH/ADMA and EGFR/ERK Signaling [Pubmed:29523851]
Mechanisms of Ageing and Development Multivessel analysis of progressive vascular aging in the rat: Asynchronous vulnerability among vascular territories [Pubmed:29605639]
Nutrition, Metabolism and Cardiovascular Diseases Arginine bioavailability and endothelin-1 system in the regulation of vascular function of umbilical vein endothelial cells from intrauterine growth restricted newborns [Doi: 10.1016/j.numecd.2018.09.002]
Biochemical and Biophysical Research Communications Probucol improves erectile function via Activation of Nrf2 and coordinates the HO-1/DDAH/PPAR-γ/eNOS pathways in streptozotocin-induced diabetic rats [Pubmed: 30454888]
Brazilian Journal of Pharmaceutical Science Demographic, clinical and lifestyle predictors for severity of erectile dysfunction and biomarkers level in Malaysian patients []
ACS Combinatorial Science Novel cellularly active inhibitor regresses DDAH1 induced prostate tumor growth by restraining tumor angiogenesis through targeting DDAH1/ADMA/NOS pathway [Pubmed: 30673277]
International Journal of the Cardiovascular Academy Nebivolol prevents the increase of asymmetric dimethylarginine and oxidants in hyperhomocysteinemic rats []
Oxid Med Cell Longev The Role of Reactive Oxygen Species and Nitric Oxide in the Inhibition of Trichophyton rubrum Growth by HaCaT Cells [Pubmed: 32104540]
Molecular Medicine Reports Blueberry anthocyanin‑enriched extract ameliorates transverse aortic constriction‑induced myocardial dysfunction via the DDAH1/ADMA/NO signaling pathway in … [Pubmed: 31746378]
International Journal of Nephrology Effect of a Supervised Peridialytic Exercise Program on Serum Asymmetric Dimethylarginine in Maintenance Hemodialysis Patients [Pubmed: 33163233]
Journal of Clinical Medicine The Usefulness of Serum Biomarkers in the Early Stages of Diabetic Retinopathy: Results of the EUROCONDOR Clinical Trial [Pubmed: 32344735]
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub Levels of endothelial substances in patients with newly identified hypertension compared with healthy controls [33463628]
WIENER KLINISCHE WOCHENSCHRIFT Non-functioning adrenal incidentalomas may increase toxic metabolites [34255169]
pharmaceuticals Effect of a Low Dose of Carvedilol on Cyclophosphamide-Induced Urinary Toxicity in Rats—A Comparison with Mesna [34959638]
Int J Biochem Cell Biol HIF-1α and Nrf2 regulates hypoxia induced overexpression of DDAH1 through promoter activation in prostate cancer [Pubmed:35644470]
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