转化生长因子β3(TGFb3)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Transforming Growth Factor Beta 3 (TGFb3)

TGF-B3; LAP; Latency-associated peptide

  • 转化生长因子β3(TGFb3)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 转化生长因子β3(TGFb3)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对转化生长因子β3(TGFb3)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

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参考文献

杂志 参考文献
PLoS ONE A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches [Plosone: Source]
J Reprod Immunol The immunomodulating effect of seminal plasma on T cells [PubMed: 25799173]
Fertility and Sterility Influence of vitamin D and transforming growth factor β3 serum concentrations, obesity, and family history on the risk for uterine fibroids [pubmed:27743697]
Eur J Pharm Biopharm New scaffolds encapsulating TGF-β3/BMP-7 combinations driving strong chondrogenic differentiation [pubmed:28087378]
Transforme edici büyüme faktörü-beta-1 nötralizan antikoru ve transforme edici büyüme faktörü-beta-3'ün trakeal darlık gelişimi üzerine etkileri [10.5606/tgkdc.dergisi.2017.14302]
Fertility and Sterility Ulipristal acetate decreases transforming growth factor β3 serum and tumor tissue concentrations in patients with uterine fibroids [Pubmed:29525690]
Scientific Reports TGF-β concentrations and activity are down-regulated in the aqueous humor of patients with neovascular age-related macular degeneration [Pubmed:29795291]
Immunology Letters The involvement of multifunctional TGF-β and related cytokines in pathogenesis of endometriosis [Pubmed: 30367890]
Cell Reports Transforming Growth Factor-β3 Regulates Adipocyte Number in Subcutaneous White Adipose Tissue [Pubmed: 30332637]
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Temporal TGF-β Supergene Family Signalling Cues Modulating Tissue Morphogenesis: Chondrogenesis within a Muscle Tissue Model? [Pubmed: 32660137]
Equine Vet J Limited added value of negative pressure wound therapy compared to calcium alginate dressings for second intention healing in a non©\contaminated and?¡­ [34115409]
AAPS PharmSciTech Nanoparticles and Nanostructured Films with TGF-β3: Preparation, Characterization, and Efficacy [34378118]
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