抗C反应蛋白抗体(Anti-CRP)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Anti-C Reactive Protein Antibody (Anti-CRP)

C-RP; PTX1; Pentraxin-Related; Pentraxin 1

  • 抗C反应蛋白抗体(Anti-CRP)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 抗C反应蛋白抗体(Anti-CRP)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 抗C反应蛋白抗体(Anti-CRP)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • AEA821Hu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测抗C反应蛋白抗体(Anti-CRP),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的抗C反应蛋白抗体(Anti-CRP)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 97-105 101
EDTA plasma(n=5) 81-91 85
heparin plasma(n=5) 82-95 90

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的抗C反应蛋白抗体(Anti-CRP),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中抗C反应蛋白抗体(Anti-CRP)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 95-103% 99-105% 86-95% 79-99%
EDTA plasma(n=5) 89-96% 84-101% 80-92% 78-94%
heparin plasma(n=5) 88-102% 81-103% 85-101% 85-94%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100μL,37°C孵育1小时;
4. 洗板5次;
5. 加TMB底物90µL,37°C孵育10-20分钟;
6. 加终止液50µL,立即450nm读数。

实验原理

将抗原包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的抗C反应蛋白抗体(Anti-CRP)与连接于固相载体上的抗原结合,然后加入HRP标记的二抗,彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的抗C反应蛋白抗体(Anti-CRP)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
APA821Hu01 C反应蛋白(CRP)活性蛋白 Cell culture; Activity Assays.
NPA821Hu02 C反应蛋白(CRP)天然蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
NPA821Hu01 C反应蛋白(CRP)天然蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA821Hu01 C反应蛋白(CRP)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA821Hu02 C反应蛋白(CRP)多克隆抗体 WB; IHC; ICC; IP.
PAA821Hu01 C反应蛋白(CRP)多克隆抗体 WB; IHC; ICC; IP.
LAA821Hu71 C反应蛋白(CRP)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA821Hu21 C反应蛋白(CRP)单克隆抗体 WB; IHC; ICC; IP.
RAA821Hu21 C反应蛋白(CRP)重组抗体 WB; IFC; IHC; IP.
MAA821Hu22 C反应蛋白(CRP)单克隆抗体 WB; IHC; ICC; IP.
LAA821Hu72 C反应蛋白(CRP)单克隆抗体(生物素标记) WB; IHC; ICC.
HEA821Hu C反应蛋白(CRP)检测试剂盒(酶联免疫吸附试验法,高敏型) Enzyme-linked immunosorbent assay for Antigen Detection.
SEA821Hu C反应蛋白(CRP)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
AEA821Hu 抗C反应蛋白抗体(Anti-CRP)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antibody Detection.
SCA821Hu C反应蛋白(CRP)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
PSA821Hu01 C反应蛋白(CRP)抗体对 ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA821Hu01 C反应蛋白(CRP)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Journalof Vascular Research Effect of Hirulog-Like Peptide on Balloon Catheter Injury-Induced Neointimal Formation in Femoral Arteries of Minipigs and Relationship with Inflammatory Mediators [Karger: 000257340]
Journal of Pharmacology and Experimental Therapeutics Successful Treatment of Acute Lung Injury with Pitavastatin in Septic Mice: Potential Role of Glucocorticoid Receptor Expression in Alveolar Macrophages [Aspet: 171462]
Experimental Animals Anti-Inflammatory Effect of Guan-Xin-Er-Hao via the Nuclear Factor-Kappa B Signaling Pathway in Rats with Acute Myocardial Infarction [Jstage: source]
Neuroscience In vivo protective effect of ferulic acid against noise-induced hearing loss in the guinea-pig [PubMed: 20600667]
Heart and Vessels Metformin inhibits nuclear factor κB activation and decreases serum high-sensitivity C-reactive protein level in experimental atherogenesis of rabbits [SpringerLink: pt732478620w1528]
Plos one Olive (Olea europaea L.) Leaf Polyphenols Improve Insulin Sensitivity in Middle-Aged Overweight Men: A Randomized, Placebo-Controlled, Crossover Trial [PubMed: 23516412]
Psychosomatic Medicine Unpredictable chronic mild stress promotes atherosclerosis in high cholesterol-fed rabbits [PubMed: 22753638]
PLOS ONE Protective Effect of Curcumin on Pulmonary and Cardiovascular Effects Induced by Repeated Exposure to Diesel Exhaust Particles in Mice [PlosOne: Source]
BMC Neurology Relationships among serum C-reactive protein, receptor for advanced glycation products, metabolic dysfunction, and cognitive impairments [PubMed: PMC3765839]
Particle and fibre toxicology, Impact of experimental type 1 diabetes mellitus on systemic and coagulation vulnerability in mice acutely exposed to diesel exhaust particles [PubMed: PMC3641025]
Clinics Changes in body weight, C-reactive protein, and total adiponectin in non-obese women after 12 months of a small-volume, home-based exercise program [PubMed: PMC3752622]
PLoS One Pre-Pubertal Children Born Post-Term Have Reduced Insulin Sensitivity and Other Markers of the Metabolic Syndrome [PubMed: PMC3698136]
Scientific Report Among overweight middle-aged men, first-borns have lower insulin sensitivity than second-borns [Pubmed: 24503677]
International Urology Nephrology Perirenal fat associated with microalbuminuria in obese rats [Pubmed: 24526332]
AJBM Effect of caffeic acid on doxorubicin induced cardiotoxicity in rats [Ajbm: Source]
Journal of Molecular and Cellular Cardiology Effect of high free fatty acids on the anti-contractile response of perivascular adipose tissue in rat aorta [Pubmed: 23939490]
PLoS One. Flos Lonicera Ameliorates Obesity and Associated Endotoxemia in Rats through Modulation of Gut Permeability and Intestinal Microbiota [Plos: Source]
Fundamental & Clinical Pharmacology The determination of histopathological and biochemical effects of the rabbit knee joint injected dexketoprofen trometamol [Onlinelibrary: fcp.12074]
Mainz Identifizierung neuer potentieller Biomarker für das Kolonkarzinom sowie dessen Vorstufen [Info:Source]
Int J Clin Exp Med. Celastrol may have an anti-atherosclerosis effect in a rabbit experimental carotid atherosclerosis model [Pubmed:Pmc4132129]
International Urology and Nephrology As If Biomarker Discovery Isn&#039;t Hard Enough: The Consequences of Poorly Characterized Reagents [Pubmed:24305687]
Nutr Res. Curcumin attenuates adhesion molecules and matrix metalloproteinase expression in hypercholesterolemic rabbits [Pubmed:25282128]
Journal of Obstetrics and Gynaecology Research Regression of experimental endometriotic implants in a rat model with the angiotensin II receptor blocker losartan [Pubmed:25302540]
Chemical Research in Toxicology Sub-chronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwall Carbon Nanotubes by Intratracheal Instillation [Pubmed:25580880]
Journal of Applied Toxicology Bisphenol A exposure induces metabolic disorders and enhances atherosclerosis in hyperlipidemic rabbits [Pubmed:25619500]
Cellular Physiology and Biochemistry Short-Term Nose-Only Water-Pipe (Shisha) Smoking Exposure Accelerates Coagulation and Causes Cardiac Inflammation and Oxidative Stress in Mice [Pubmed:25634761]
Food and Chemical Toxicology Consumption of orange fermented beverage reduces cardiovascular risk factors in healthy mice [Pubmed:25666657]
Cellular Physiology and Biochemistry Induction of Haemeoxygenase-1 Improves FFA-Induced Endothelial Dysfunction in Rat Aorta [Pubmed:25766533]
天津中医药大学学报 巨噬细胞及其相关因子在家兔不稳定斑块中的作用及其机制 [Paper:Source]
Mediators of Inflammation Effects of Intravenous Injection of [PubMed: 26063970]
Am J Physiol Heart Circ Physiol Irisin improves endothelial function in obese mice through the AMPK-eNOS pathway [PubMed: 26371167]
Cell Physiol Biochem Induction of Haemeoxygenase-1 Directly Improves Endothelial Function in Isolated Aortas from Obese Rats through the Ampk-Pi3k/Akt-Enos Pathway [PubMed: 26160485]
Exp Physiol Renal protective effects of induction of haem oxygenase-1 combined with increased adiponectin on the glomerular vascular endothelial growth factor–nitric oxide axis in obese rats [PubMed: 25959017]
Clin Oral Investig Can apical periodontitis affect serum levels of CRP, IL-2, and IL-6 as well as induce pathological changes in remote organs? [PubMed: 26556576]
Chemical Research in Toxicology Subchronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwalled Carbon Nanotubes by Intratracheal Instillation [PubMed: 25580880]
Acta Biomater Resveratrol Inhibited Inflammation andAlveolar Bone Loss in Periodontitis [PubMed: 26497626]
Archives of Physiology and Biochemistry Activation of cardiac renin–angiotensin system and plasminogen activator inhibitor-1 gene expressions in oral contraceptive-induced cardiometabolic disorder [Pubmed:26934364]
Journal of Functional Foods Orange beverage ameliorates high-fat-diet-induced metabolic disorder in mice [article:S1756464616300688]
Medical Science Monitor Variation of Circulating Inflammatory Mediators in Staphylococcus aureus and Escherichia coli BloodstreamInfection. [Pubmed:26772168]
J Diabetes Res.  Central Administration of Galanin Receptor 1 Agonist Boosted Insulin Sensitivity in Adipose Cells of Diabetic Rats [pmc:PMC4835658]
Asian J Pharm Clin Res THE ANTI-HYPERCHOLESTEROLEMIC EFFECT OF ULVAN POLYSACCHARIDE EXTRACTED FROM THE GREEN ALGA ULVAFASCIATA ON AGED HYPERCHOLESTEROLEMIC RATS [journals:10835]
J Bone Miner Res Fat and Sucrose Intake Induces Obesity‐Related Bone Metabolism Disturbances: Kinetic and Reversibility Studies in Growing and Adult Rats [Pubmed:26175082]
Naunyn-Schmiedeberg's Archives of Pharmacology Combined oral contraceptive-induced hypertension is accompanied by endothelial dysfunction and upregulated intrarenal angiotensin II type 1 receptor gene expression [pubmed:27447455]
Journal of Coastal Life Medicine Cardioprotective and renoprotective effects of Cocos nucifera water in offspring of high fat diet fed Wistar rat dams [qk:20168]
Journal of Functional Foods Eicosapentaenoic acid-enriched phospholipids improve atherosclerosis by mediating cholesterol metabolism [S1756464617300890]
Environmental toxicology and pharmacology Combined oral contraceptive and nitric oxide synthesis inhibition synergistically causes cardiac hypertrophy and exacerbates insulin resistance in female rats. [pubmed:28376377]
Andrology Chronic prostatitis/chronic pelvic pain syndrome impairs erectile function through increased endothelial dysfunction, oxidative stress, apoptosis, and corporal fibrosis … [doi:10.1111]
Journal of Diabetes Research Central administration of galanin receptor 1 agonist boosted insulin sensitivity in adipose [pubmed:27127795]
Therapeutics and Clinical Risk Management Elevated blood plasma levels of epinephrine, norepinephrine, tyrosine hydroxylase, TGFβ1, and TNFα associated with high-altitude pulmonary edema in an Indian population. [pubmed:27540296]
Veterinary World Acute phase response in lame crossbred dairy cattle [pubmed:27956769]
J Ethnopharmacol Anti-atherosclerosis and cardio-protective effects of the Angong Niuhuang Pill on a high fat and vitamin D3 induced rodent model of atherosclerosis. [pubmed:27880884]
SHOCK INTERLEUKIN-1 RECEPTOR 2: A NEW BIOMARKER FOR SEPSIS DIAGNOSIS AND GRAM-NEGATIVE/GRAM-POSITIVE BACTERIAL DIFFERENTIATION [pubmed:27984536]
International Surgery PROCALCITONIN, C-REACTIVE PROTEIN AND FIBRINOGEN LEVELS IN CASES WITH MECHANIC BOWEL OBSTRUCTION AND THEIR CHANGE IN RELATION TO THE DURATION OF OBSTRUCTION: AN EXPERIMENTAL STUDY [10.3892/ol.2017.7301]
CORONARY ARTERY DISEASE Relationship of mean platelet volume to lymphocyte ratio and coronary collateral circulation in patients with stable angina pectoris [pubmed:28678144]
Biomedicine & Pharmacotherapy Protective effect of α–lipoic acid against spleen toxicity of dimethylnitrosamine in male mice: Antioxidant and ultrastructure approaches [pubmed:29031205]
PLoS One The effects of non-surgical periodontal treatment on glycemic control, oxidative stress balance and quality of life in patients with type 2 diabetes: A randomized clinical trial [pubmed:29145468]
Poultry Science The effect of DL-, L-isomers and DL-hydroxy analog administered at 2 levels as dietary sources of methionine on the metabolic and antioxidant parameters and growth performance of turkeys [10.3382/ps/pex099]
Oncotarget The immune impact of mimic endoscopic retrograde appendicitis therapy and appendectomy on rabbits of acute appendicitis. [pubmed:29029533]
International Journal of Cuban Health & Medicine Anemia and Iron Deficiency Related to Inflammation, Helicobacter pylori Infection and Adiposity in Reproductive-age Cuban Women [10.1590/MEDICC.2017.1902030004 ]
Korean Journal of Internal Medicine The relationship between anti-C-reactive protein and disease activity in patients with systemic lupus erythematosus. [pubmed:28352058]
Biomedical and Environmental Sciences Grape Seed Procyanidin Extract Reduces Arsenic-Induced Renal Inflammatory Injury in Male Mice. [pubmed:28756814]
Pharmacological reports Anti-inflammatory and antithrombotic effects of nicotine exposure in oral contraceptive-induced insulin resistance are glucocorticoid-independent [pubmed:28349880]
Nature Communications A ligand-specific blockade of the integrin Mac-1 selectively targets pathologic inflammation while maintaining protective host-defense [Pubmed:29410422]
International Journal of Obesity Leptin alleviates intestinal mucosal barrier injury and inflammation in obese mice with acute pancreatitis [Pubmed:29934609]
Molecular Nutrition & Food Research The Protective Activities of Dietary Sea Cucumber Cerebrosides Against Atherosclerosis Through Regulating Inflammation and Cholesterol Metabolism in Male Mice [Pubmed:29883529]
Oxidative Medicine and Cellular Longevity Changes of Plasma FABP4, CRP, Leptin, and Chemerin Levels in relation to Different Dietary Patterns and Duodenal-Jejunal Omega Switch Surgery in Sprague … [Pubmed:29849871]
Nutrients L-Carnitine Supplementation in Older Women. A Pilot Study on Aging Skeletal Muscle Mass and Function [Pubmed:29473908]
Journal of Functional Foods Saponins of sea cucumber attenuate atherosclerosis in ApoE−/− mice via lipid-lowering and anti-inflammatory properties [10.1016:j.jff.2018.07.046]
Veterinary immunology and immunopathology Generation and validation of novel anti-bovine CD163 monoclonal antibodies ABM-1A9 and ABM-2D6 [Pubmed:29571519]
Journal of Experimental Animal Science  Oxymatrine attenuates lipopolysaccharide-induced acute lung injury by activating the epithelial sodium channel and suppressing the JNK signaling pathway [Pubmed:29526865]
Journal of Young Pharmacists Anti-Inflammatory Activity of Combined Aerodisperse System in the Conditions of Experimental Cervical Lymphadenite [:]
Journal of Pharmacological Sciences Improved endogenous epoxyeicosatrienoic acid production mends heart function via increased PGC 1α-mitochondrial functions in metabolic syndrome [Pubmed: 30342783]
The Egyptian Rheumatologist Sonographic synovitis in primary knee osteoarthritis patients and its relation to biomarkers of inflammation and oxidative stress [Doi: 10.1016/j.ejr.2018.12.004]
West Indian Medical Journal Plasma Cellular Hypoxia, Mitochondrial Dysfunction, Disease Risk and Prognostic Factors in Type 2 Diabetic Patients in Saudi Arabia. []
Nutrition & Metabolism L-Carnitine Supplementation Increases Trimethylamine-N-Oxide but not Markers of Atherosclerosis in Healthy Aged Women [Pubmed: 30485835]
The Egyptian Rheumatologist The Egyptian Rheumatologist []
Experimental Biology and Medicine Grape seed proanthocyanidin extract alleviates arsenic-induced lung damage through NF-κB signaling [Pubmed: 30869553]
International Journal of Inflammation Morphological Characteristics of the Thymus and Spleen and the Subpopulation Composition of Lymphocytes in Peripheral Blood during Systemic Inflammatory … []
Journal of Central European Agriculture Novel assay for the detection of CRP protein in rabbit leukocytes using flow cytometry []
Journal of Clinical Medicine Periodontal Health and Oral Microbiota in Patients with Rheumatoid Arthritis [Pubmed: 31072030]
Journal of Applied Pharmaceutical Science Effects of Eurycoma longifolia Jack on chronic cerebral hypoperfusion-induced oxidative damage and memory deficit in rats []
Food & Function Eicosapentaenoic acid in the form of phospholipid exerts superior anti-atherosclerosis effects than its triglyceride form in ApoE-/-mice []
Journal of Central European Agriculture Novel assay for the detection of CRP protein in rabbit leukocytes using flow cytometry/Nova metoda detekcie CRP proteinu na kralicich leukocytoch pomocou … []
留言咨询