γ-氨基丁酸(gABA)等多因子检测试剂盒(流式荧光发光法)

Multiplex Assay Kit for Gamma-Aminobutyric Acid (gABA) ,etc. by FLIA (Flow Luminescence Immunoassay)

4-Aminobutyric Acid

（注：单次混测多因子不超过8个指标 ）

  • γ-氨基丁酸(gABA)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • γ-氨基丁酸(gABA)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测γ-氨基丁酸(gABA)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的γ-氨基丁酸(gABA)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 96-105 101
EDTA plasma(n=5) 82-101 97
heparin plasma(n=5) 86-101 93

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

线性

在定值血清及血浆样本内加入适量的γ-氨基丁酸(gABA)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中γ-氨基丁酸(gABA)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 96-104% 95-105% 86-96% 89-98%
EDTA plasma(n=5) 96-105% 91-98% 80-98% 83-98%
heparin plasma(n=5) 94-105% 78-101% 80-91% 87-95%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠、检测溶液A）标准品或样本50μL及磁珠10μL，加检测溶液A50μL，
    37°C酶标板振荡器孵育60分钟；
3. 磁吸洗板3次；
4. 加检测溶液B100μL，37°C振动孵育30分钟；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋涡震荡2分钟后读数。

实验原理

将γ-氨基丁酸(gABA)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠、标记VEGFA，标记的γ-氨基丁酸(gABA)等多因子检测试剂盒(流式荧光发光法)和未标记的γ-氨基丁酸(gABA)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体竞争结合，然后将未结合物洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的γ-氨基丁酸(gABA)等多因子检测试剂盒(流式荧光发光法)呈负相关。

赠品

相关产品

编号 适用物种：Pan-species (General，通用) 应用(仅供研究使用，不用于临床诊断！)
CPA900Ge11 γ-氨基丁酸(gABA)牛血清白蛋白偶联物 Immunogen; SDS-PAGE; WB.
CPA900Ge21 γ-氨基丁酸(gABA)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
CPA900Ge41 γ-氨基丁酸(gABA)人血清白蛋白偶联物 Immunogen; SDS-PAGE; WB.
PAA900Ge01 γ-氨基丁酸(gABA)多克隆抗体 ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
PAA900Ge02 γ-氨基丁酸(gABA)多克隆抗体 ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
LAA900Ge71 γ-氨基丁酸(gABA)多克隆抗体(生物素标记) WB; IHC; ICC.
LAA900Ge81 γ-氨基丁酸(gABA)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
CEA900Ge γ-氨基丁酸(gABA)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
AEA900Ge 抗γ-氨基丁酸抗体(Anti-gABA)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antibody Detection.
LMA900Ge γ-氨基丁酸(gABA)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA900Ge11 γ-氨基丁酸(gABA)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Toxicology and Industrial Health Effect of sub-acute exposure to acrylamide on GABAergic neurons and astrocytes in weaning rat cerebellum [PubMed: 21444355]
Neurosci Lett Expression of the neuron-specific potassium chloride cotransporter KCC2 in adult rat cochlear. [PubMed: 18577424]
Biomark Insights Decreased Epidermal Growth Factor (EGF) Associated with HMGB1 and Increased Hyperactivity in Children with Autism [PubMed: PMC3623607]
Biomarker insights Correlation Between Hepatocyte Growth Factor (HGF) and Gamma-Aminobutyric Acid (GABA) Plasma Levels in Autistic Children [PubMed: PMC3694825]
Biomarker insights Decreased Hepatocyte Growth Factor (HGF) and Gamma Aminobutyric Acid (GABA) in Individuals with Obsessive-Compulsive Disorder (OCD) [PubMed: PMC3762604]
Scientific Reports Renal Denervation Improves the Baroreflex and GABA System in Chronic Kidney Disease-induced Hypertension [pubmed:27917928]
Journal of Neuroscience Sex differences in the glutamate signaling pathway in juvenile rats [Pubmed:28861894]
Scientific Reports A novel homozygous mutation in GAD1 gene described in a schizophrenic patient impairs activity and dimerization of GAD67 enzyme [Pubmed: 30341396]
Neurochemical Research Aucubin Alleviates Seizures Activity in Li-Pilocarpine-Induced Epileptic Mice: Involvement of Inhibition of Neuroinflammation and Regulation of Neurotransmission [Pubmed: 30666488]
Neuroscience Letters Differentiation of human glioblastoma U87 cells into cholinergic neuron [Pubmed: 30928478]
Neurochemical Journal Glutamate Signaling Defects in Propionic Acid Orally Administered to Juvenile Rats as an Experimental Animal Model of Autism []
Effect of gamma radiation on combination therapy of certain antiepileptic drugs in rats. []
Experimental and Therapeutic Medicine Antiepileptic effects of exogenous β‑hydroxybutyrate on kainic acid‑induced epilepsy [Pubmed: 33101467]
Exp Anim Dementia model mice exhibited improvements of neuropsychiatric symptoms as well as cognitive dysfunction with neural cell transplantation [33828024]
Neurogenetics Age-dependent neurological phenotypes in a mouse model of PRRT2-related diseases [34101060]
Sci Rep Overexpression of wild-type human amyloid precursor protein alters GABAergic transmission [34475508]
J Food Biochem Adaptogenic potential of ginsenosides against domoic acid‐induced toxicity by regulating neuronal stress and kinate receptors: Ex vivo and in silico studies [Pubmed:35102569]
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