抗增殖细胞核抗原(PCNA)多克隆抗体
Polyclonal Antibody to Proliferating Cell Nuclear Antigen (PCNA)
Cyclin
- 编号PAA591Mi01
- 物种Homo sapiens (Human，人), Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠), Oryctolagus cuniculus (Rabbit，兔), Rhesus monkey (Simian，恒河猴), Canis familiaris; Canine (Dog，犬), Sus scrofa; Porcine (Pig，猪) 相同的名称，不同的物种。
- 来源多抗制备
- 宿主兔
- 效价-
- Ig类型 IgG
- 纯化方式抗原特异性亲和纯化
- 标记物无标记物
- 免疫原 RPA591Mi01-抗增殖细胞核抗原(PCNA)重组蛋白
- 缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
- 性状液体
- 浓度0.62mg/ml
- 且适物种Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠), Cavia (Guinea pig ，豚鼠), Canis familiaris; Canine (Dog，犬), Sus scrofa; Porcine (Pig，猪), Bos taurus; Bovine (Cattle，牛), Capra hircus; Caprine (Goat，山羊), Ovis aries; Ovine (Sheep，绵羊), Equus caballus; Equine (Horse，马), Chicken (Gallus，鸡)
- 应用WB; IHC; ICC; IP.
如果抗体需用于流式细胞术，请参见流式抗体。 - 下载 英文说明书 中文说明书
- 规格 20µl100µl 200µl 1ml 10ml
- 价格 ¥ 662 ¥ 1546 ¥ 2208 ¥ 5520 ¥ 22080
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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DAB staining on IHC-P; Samples: Human Colorectal cancer Tissue; Primary Ab: 20µg/ml Rabbit Anti-Multi-species PCNA Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Human Thyroid cancer Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human PCNA Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Liver Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human PCNA Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Stomach Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human PCNA Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Mammary gland Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human PCNA Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Tonsil Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human PCNA Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Figure. Western Blot; Sample: Recombinant PCNA.
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Western Blot; Sample: Lane1: Porcine Liver lysate; Lane2: Porcine Cerebrum lysate; Lane3: Porcine Heart lysate; Lane4: Bovine Liver lysate; Lane5: Bovine Cerebrum lysate; Lane6: Bovine Heart lysate; Lane7: Caprine Cerebrum lysate
Primary Ab: 3µg/mL Rabbit Anti-Multi-species PCNA Ab
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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通过ISO 9001、ISO 13485质量体系认证
特异性
该抗体是针对PCNA的兔多克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别PCNA。
用法
Western blotting: 0.5-3µg/mL;
Immunohistochemistry: 5-30µg/mL;
Immunocytochemistry: 5-30µg/mL;
Optimal working dilutions must be determined by end user.
储存
经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
赠品
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人), Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠), Oryctolagus cuniculus (Rabbit，兔), Rhesus monkey (Simian，恒河猴), Canis familiaris; Canine (Dog，犬), Sus scrofa; Porcine (Pig，猪) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA591Mi01 | 抗增殖细胞核抗原(PCNA)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA591Mi01 | 抗增殖细胞核抗原(PCNA)多克隆抗体 | WB; IHC; ICC; IP. |
| MAA591Mi22 | 抗增殖细胞核抗原(PCNA)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA591Mi23 | 抗增殖细胞核抗原(PCNA)单克隆抗体 | WB; IHC; ICC; IP. |
| CAA591Mi22 | 抗抗增殖细胞核抗原(PCNA)单克隆抗体 | Loading Control of WB |
| MAA591Mi24 | 抗增殖细胞核抗原(PCNA)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA591Mi21 | 抗增殖细胞核抗原(PCNA)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA591Mi | 抗增殖细胞核抗原(PCNA)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA591Mi | 抗增殖细胞核抗原(PCNA)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Tissue and Cell | Human umbilical cord blood-mesenchymal stem cells transplantation renovates the ovarian surface epithelium in a rat model of premature ovarian failure: Possible direct and indirect effects [Pubmed:27233913] |
| Food and Chemical Toxicology | Antioxidant, anti-inflammatory and synergistic anti-hyperglycemic effects of Malaysian propolis and metformin in streptozotocin–induced diabetic rats [Pubmed:30026088] |
| Life Sciences | Malaysian propolis, metformin and their combination, exert hepatoprotective effect in streptozotocin-induced diabetic rats [Pubmed: 30205096] |
| Archives of Physiology and Biochemistry | Diabetes-induced testicular oxidative stress, inflammation, and caspase-dependent apoptosis: the protective role of metformin [Pubmed: 30513216] |
| European journal of medicinal chemistry | Synthesis of novel S-linked dihydroartemisinin derivatives and evaluation of their anticancer activity [Pubmed: 31216504] |
| Antioxidants (Basel). | The Effect of Dietary Quercetin on the Glutathione Redox System and Small Intestinal Functionality of Weaned Piglets [Pubmed: 31426309] |
| Antioxidants | Oxidative Stress, NF-κB-Mediated Inflammation and Apoptosis in the Testes of Streptozotocin–Induced Diabetic Rats: Combined Protective Effects of Malaysian … [Pubmed: 31600920] |
| TOXICOLOGY | Tert-butylhydroquinone preserve testicular steroidogenesis and spermatogenesis in cisplatin-intoxicated rats by targeting oxidative stress, inflammation and apoptosis [Pubmed: 32565124] |
| BIOMEDICINE & PHARMACOTHERAPY | Protective effects of bee bread on testicular oxidative stress, NF-κB-mediated inflammation, apoptosis and lactate transport decline in obese male rats [Pubmed: 33152939] |
| Sci Rep | Tert-butylhydroquinone attenuates doxorubicin-induced dysregulation of testicular cytoprotective and steroidogenic genes, and improves spermatogenesis in rats [33750916] |
| J Cell Sci | Local DNA synthesis is critical for DNA repair during oocyte maturation [34415018] |
| Stem Cell Rev Rep | Comparative Evaluation of Anti-Fibrotic Effect of Tissue Specific Mesenchymal Stem Cells Derived Extracellular Vesicles for the Amelioration of CCl4 Induced Chronic … [34859376] |
| Naunyn-Schmiedeberg's Archives | Inflammatory Modulation of miR-155 Inhibits Doxorubicin‑Induced Testicular Dysfunction Via Sirt1/Foxo1 Pathway: Insight to the Role of Acacetin and Bacillus … [Pubmed:35715546] |
| Antioxidants | Therapeutic Effects of Bee Bread on Obesity-Induced Testicular-Derived Oxidative Stress, Inflammation, and Apoptosis in High-Fat Diet Obese Rat Model … [Pubmed:35204140] |
| Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β … [] | |
| Impact de l'incorporation d'acides gras à chaine moyenne, de suppléments de levures et d'avoine nue sur la santé intestinale ainsi que sur la consommation … [] |
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