摄食抑制因子1(NES1)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Nesfatin 1 (NES1)
- 编号CEA242Ra
- 物种Rattus norvegicus (Rat,大鼠) 相同的名称,不同的物种。
- 实验方法竞争抑制
- 反应时长2h
- 检测范围493.8-40,000pg/mL
- 灵敏度最小可检测剂量小于等于177.4pg/mL.
- 样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 2873 ¥ 4104 ¥ 18468 ¥ 34884 ¥ 287280
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特异性
本试剂盒用于检测摄食抑制因子1(NES1),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的摄食抑制因子1(NES1)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。
样本 | 回收率范围(%) | 平均回收率(%) |
serum(n=5) | 88-96 | 92 |
EDTA plasma(n=5) | 78-104 | 83 |
heparin plasma(n=5) | 91-105 | 99 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%
线性
在定值血清及血浆样本内加入适量的摄食抑制因子1(NES1),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中摄食抑制因子1(NES1)含量的测定值与理论值的比率。
样本 | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 92-99% | 99-105% | 88-95% | 86-94% |
EDTA plasma(n=5) | 83-90% | 79-89% | 82-96% | 93-103% |
heparin plasma(n=5) | 86-94% | 80-96% | 87-103% | 93-101% |
稳定性
经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)50µL,
加入50µL检测液A(临用前配制);
37°C温育1小时。
3. 洗板3次;
4. 加检测溶液B100µL,37°C孵育30分钟;
5. 洗板5次;
6. 加TMB底物90µL,37°C孵育10-20分钟;
7. 加终止液50µL,立即450nm读数。
实验原理
本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将摄食抑制因子1(NES1)单克隆抗体包被微孔板,制成固相载体,往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原(标准品或样本),待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物,然后加入HRP标记的亲和素,经过温育和彻底洗涤后加入底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。待测标本浓度越高,标记抗原和抗体的结合就越受到抑制,显色愈浅。显色的深浅与酶量呈正相关,而与样品中待测物质含量呈负相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。
相关产品
编号 | 适用物种:Rattus norvegicus (Rat,大鼠) | 应用(仅供研究使用,不用于临床诊断!) |
EPA242Ra61 | 摄食抑制因子1(NES1)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APA242Ra01 | 摄食抑制因子1(NES1)活性蛋白 | Cell culture; Activity Assays. |
RPA242Ra01 | 摄食抑制因子1(NES1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APA242Ra61 | 摄食抑制因子1(NES1)活性蛋白 | Cell culture; Activity Assays. |
PAA242Ra01 | 摄食抑制因子1(NES1)多克隆抗体 | WB; IHC; ICC; IP. |
LAA242Ra81 | 摄食抑制因子1(NES1)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
LAA242Ra71 | 摄食抑制因子1(NES1)多克隆抗体(生物素标记) | WB; IHC; ICC. |
SEA242Ra | 摄食抑制因子1(NES1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
CEA242Ra | 摄食抑制因子1(NES1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA242Ra | 摄食抑制因子1(NES1)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
杂志 | 参考文献 |
Bulletin of Clinical Psychopharmacology | Plasma Nesfatin-1 Level May Be Associated with Disease Severity in Patients with Panic Disorder [Psikofarmakoloji: 2043] |
Progress in Neuro-Psychopharmacology & Biological Psychiatry | High plasma nesfatin-1 level in patients with major depressive disorder [PubMed: 21163317] |
Endocrine | Cord blood nesfatin-1 and apelin-36 levels in gestational diabetes mellitus [SpringerLink: h978088657555w16] |
Bulletin of Clinical Psychopharmacology | Increased Plasma Nesfatin-1 Levels in Patients with Obsessive Compulsive Disorder [Psikofarmakoloji: 2212] |
Psychoneuroendocrinology. | Decreased plasma nesfatin-1 levels in patients with generalized anxiety disorder [PubMed: 22595767] |
Iranian Journal of Health and Physical activity | Effects of Aerobic Training, with or without Zizyphus Jujuba Water Extraction, on Fundus Nesfatin-1, ATP, HDL-C, and LDL-C Concentrations in Female Rats [Ijvst: Source] |
European Journal of Obstetrics & Gynecology and Reproductive Biology | Decreased serum nesfatin-1 levels in endometriosis [Pubmed: 24702902] |
Bulletin of Clinical Psychopharmacology | Increased Plasma Nesfatin-1 Levels in Patients with Obsessive Compulsie Disorder. [Psikofarmakoloji: Source] |
Current Pharmaceutical Design | Role of NUCB2/nesfatin-1 as a possible biomarker. [Pubmed: 23537082] |
Regulatory, Integrative and Comparative Physiology | A role of nesfatin-1/NucB2 in dehydration-induced anorexia [Pubmed:24829503] |
J Endocrinol Invest. | Plasma nesfatin-1 levels are increased in patients with polycystic ovary syndrome [Pubmed:24920281] |
Int J Surg. | The potential role of BMI, plasma leptin, nesfatin-1 and ghrelin levels in the early detection of pancreatic necrosis and severe acute pancreatitis: a prospective cohort study. [Pubmed:25448651] |
Hormone molecular biology and clinical investigation | The effects of short-term high-intensity interval training vs. moderate-intensity continuous training on plasma levels of nesfatin-1 and inflammatory markers [Pubmed:25581765] |
Turk J Gastroenterol. | New hormones to predict the severity of gallstone-induced acute pancreatitis [Pubmed:25599787] |
Respiration | Is There a Relationship between Obstructive Sleep Apnea Syndrome Severity and Nesfatin-1? [PubMed: 26088934] |
J Clin Res Pediatr Endocninol | Correlation of Brain Neuropeptide (Nesfatin-1 and Orexin-A) Concentrations with Anthropometric and Biochemical Parameters in Malnourished Children [PubMed: 26831553] |
Bosn J Basic Med Sci | Nesfatin-1 and Vitamin D levels may be associated with systolic and diastolic blood pressure values and hearth rate in polycystic ovary syndrome [PubMed: 26295295] |
Original Article / Orijinal Makale | Relationship and Interaction between Anaerobic Sports Branches and Serum Nesfatin-1 [Sayilar: 294] |
Gynecol Endocrinol | Nesfatin-1 levels and metabolic markers in polycystic ovary syndrome [PubMed: 26062107] |
International Journal of Neuroscience | Serum nesfatin-1 levels: a potential new biomarker in patients with subarachnoid hemorrhage* [Pubmed:26872406] |
未找到 | Comparing the nesfatin-1 levels between individuals with subjective cognitive decline and mild cognitive impairment [P.1.e.012.pdf] |
International Journal of Developmental | Serum nesfatin-1 levels: a potential new biomarker in patients with subarachnoid hemorrhage. [pubmed:26872406] |
Current Pharmaceutical Design | Metabolic and Cardiovascular Actions of Nesfatin-1: Implications in Health and Disease [pubmed:28137218] |
Arch Endocrinol Metab | Serum nesfatin-1 levels are decreased in pregnant women newly diagnosed with gestational diabetes [pubmed:28977160] |
Indian Journal of Psychiatry | Serum NUCB2/nesfatin-1 levels in different stages of alcohol dependence: Is there a relationship with craving? [pubmed:28529367] |
Biomedicine & Pharmacotherapy | Nesfatin-1 promotes VSMC migration and neointimal hyperplasia by upregulating matrix metalloproteinases and downregulating PPARγ [Pubmed:29604590] |
Biochimica et Biophysica Acta | Nesfatin-1 functions as a switch for phenotype transformation and proliferation of VSMCs in hypertensive vascular remodeling [Pubmed:29627363] |
BMC Psychiatry | Plasma nesfatin-1 level is associated with severity of depression in Chinese depressive patients [Pubmed:29615007] |
Probiotics and Antimicrobial Proteins | Mitra Zarrati, Mahsa Raji Lahiji, Eisa Salehi, Bahareh Yazdani, Elham Razmpoosh, Raheleh Shokouhi Shoormasti & Farzad Shidfar [Pubmed: 30232744] |
An elevated serum level of nesfatin-1 may predict poor outcomes in patients with aneurysmal subarachnoid hemorrhages [] | |
clinical neurology and neurosurgery | Relationshıp of spontaneous subarachnoid haemorrhage and cerebral aneurysm to serum Visfatin and Nesfatin-1 levels [Pubmed: 32311618] |
Ann Nutr Metab | Copeptin and Nesfatin-1 Are Interrelated Biomarkers with Roles in the Pathogenesis of Insulin Resistance in Chinese Children with Obesity [Pubmed: 33027789] |
Endokrynologia Polska | Assessing the blood concentration of new adipocytokines in patients with ischaemic stroke [33283259] |
Increased serum nesfatin-1 levels among adolescents diagnosed with major depressive disorder [] |