M2-型丙酮酸激酶(PKM2)多克隆抗体

Polyclonal Antibody to Pyruvate Kinase, Muscle (PKM2)

M2PK; PKM; M2-PK; PKM1; CTHBP; OIP3; PK3; PKM; TCB; THBP1; Pyruvate kinase muscle isozyme; Thyroid hormone-binding protein 1; Cytosolic thyroid hormone-binding protein

  • M2-型丙酮酸激酶(PKM2)多克隆抗体 产品包装(模拟)
  • M2-型丙酮酸激酶(PKM2)多克隆抗体 产品包装(模拟)
  • M2-型丙酮酸激酶(PKM2)多克隆抗体 Used in DAB staining on fromalin fixed paraffin- embedded kidney tissue
  • M2-型丙酮酸激酶(PKM2)多克隆抗体 DAB staining on IHC-P;
    Samples: Human Skin cancer Tissue
  • PAA588Hu01.jpg Figure. Western Blot; Sample: Recombinant PKM2, Human.
  • M2-型丙酮酸激酶(PKM2)多克隆抗体 Western Blot; Sample: Human K562 cell lysate;
    Primary Ab: 2µg/ml Rabbit Anti-Human PKM2 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • M2-型丙酮酸激酶(PKM2)多克隆抗体 Knockout Varification:
    Lane 1: Wild-type MCF7 cell lysate;
    Lane 2: PKM2 knockout MCF7 cell lysate;
    Predicted MW: 56,58kd
    Observed MW: 60kd
    Primary Ab: 2µg/ml Rabbit Anti-Human PKM2 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • M2-型丙酮酸激酶(PKM2)多克隆抗体 Western Blot; Sample: Human MCF7 cell lysate;
    Primary Ab: 2µg/ml Rabbit Anti-Human PKM2 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • M2-型丙酮酸激酶(PKM2)多克隆抗体 Western Blot; Sample: Human Hela cell lysate;
    Primary Ab: 2µg/ml Rabbit Anti-Human PKM2 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • M2-型丙酮酸激酶(PKM2)多克隆抗体 Western Blot; Sample: Human HepG2 cell lysate;
    Primary Ab: 2µg/ml Rabbit Anti-Human PKM2 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • M2-型丙酮酸激酶(PKM2)多克隆抗体 K562 human chronic myelogenous leukemia cell line was stained with rabbit Anti-human PKM2 Polyclonal Antibody (Catalog # PAA588Hu01, filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled green histogram), followed by APC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb15). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

该抗体是针对PKM2的兔多克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别PKM2。

用法

Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

储存

经常使用则4°C保存。-20°C保存不超过一年。避免反复冻融。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPA588Hu01 M2-型丙酮酸激酶(PKM2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA588Hu02 M2-型丙酮酸激酶(PKM2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA588Hu02 M2-型丙酮酸激酶(PKM2)多克隆抗体 WB; IHC; ICC; IP.
PAA588Hu01 M2-型丙酮酸激酶(PKM2)多克隆抗体 WB; IHC; ICC; IP.
MAA588Hu22 M2-型丙酮酸激酶(PKM2)单克隆抗体 WB; IHC; ICC; IP.
SEA588Hu M2-型丙酮酸激酶(PKM2)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.

参考文献

杂志 参考文献
Cancer Research Antigen Presentation by Dendritic Cells in Tumors Is Disrupted by Altered Metabolism that Involves Pyruvate Kinase M2 and Its Interaction with SOCS3 [AACR: 70189]
Molecular BioSystems Abnormal levels of heterogeneous nuclear ribonucleoprotein A2B1 (hnRNPA2B1) in tumour tissue and blood samples from patients diagnosed with lung cancer [Pubmed:25483567]
Tumour Biol M2 isoform of pyruvate kinase (PKM2) is upregulated in Kazakh’s ESCC and promotes proliferation and migration of ESCC cells [PubMed: 26404132]
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